Abstract
This paper illustrates high-resolution bio-imaging using a miniaturized fiber based probe system which offers axial and lateral resolutions 16 μm and 144 lp/mm, respectively. The capability of imaging cellular structures is illustrated by imaging cancerous epithelium and neuronal cells. Furthermore, this study depicts a new imaging protocol, that has the potential to avoid many of the difficulties linked with quantitative fluorescence microscopy and imaging. The proposed concepts and, the flexibility and miniaturization of the probe system are explored for single cell imaging. Further the proposed probe system is found to enable imaging of fluorescent microspheres incorporated in a micro-channel. These are expected to find potential applications in pathological and physiological assessment, as well as live-cells cultured in customized live-cell imaging chambers such as perfusion chambers, flow cells and biochips.
| Original language | English |
|---|---|
| Pages (from-to) | 354-359 |
| Number of pages | 6 |
| Journal | Journal of Medical Imaging and Health Informatics |
| Volume | 1 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Dec 2011 |
Keywords
- Cellular imaging
- Fast fourier transform
- Fiber probe system
- Flow cell
- High resolution imaging
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Health Informatics
