High resolution optical imaging of epithelial and neuronal cells

V. K. Mohankumar, Parasuraman Padmanabhan, K. Sathiyamoorthy, V. M. Murukeshan (Lead / Corresponding author), James Joseph, Kishore K. Bhakoo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This paper illustrates high-resolution bio-imaging using a miniaturized fiber based probe system which offers axial and lateral resolutions 16 μm and 144 lp/mm, respectively. The capability of imaging cellular structures is illustrated by imaging cancerous epithelium and neuronal cells. Furthermore, this study depicts a new imaging protocol, that has the potential to avoid many of the difficulties linked with quantitative fluorescence microscopy and imaging. The proposed concepts and, the flexibility and miniaturization of the probe system are explored for single cell imaging. Further the proposed probe system is found to enable imaging of fluorescent microspheres incorporated in a micro-channel. These are expected to find potential applications in pathological and physiological assessment, as well as live-cells cultured in customized live-cell imaging chambers such as perfusion chambers, flow cells and biochips.

Original languageEnglish
Pages (from-to)354-359
Number of pages6
JournalJournal of Medical Imaging and Health Informatics
Volume1
Issue number4
DOIs
Publication statusPublished - 1 Dec 2011

Keywords

  • Cellular imaging
  • Fast fourier transform
  • Fiber probe system
  • Flow cell
  • High resolution imaging

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    Mohankumar, V. K., Padmanabhan, P., Sathiyamoorthy, K., Murukeshan, V. M., Joseph, J., & Bhakoo, K. K. (2011). High resolution optical imaging of epithelial and neuronal cells. Journal of Medical Imaging and Health Informatics, 1(4), 354-359. https://doi.org/10.1166/jmihi.2011.1053