High-resolution transcriptional analysis of the regulatory influence of cell-to-cell signalling reveals novel genes that contribute to Xanthomonas phytopathogenesis

Shi-Qi An, Melanie Febrer, Yvonne McCarthy, Dong-Jie Tang, Leah Clissold, Gemy Kaithakottil, David Swarbreck, Ji-Liang Tang, Jane Rogers, J. Maxwell Dow, Robert P. Ryan (Lead / Corresponding author)

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    Abstract

    The bacterium Xanthomonas campestris is an economically important pathogen of many crop species and a model for the study of bacterial phytopathogenesis. In X.?campestris, a regulatory system mediated by the signal molecule DSF controls virulence to plants. The synthesis and recognition of the DSF signal depends upon different Rpf proteins. DSF signal generation requires RpfF whereas signal perception and transduction depends upon a system comprising the sensor RpfC and regulator RpfG. Here we have addressed the action and role of Rpf/DSF signalling in phytopathogenesis by high-resolution transcriptional analysis coupled to functional genomics. We detected transcripts for many genes that were unidentified by previous computational analysis of the genome sequence. Novel transcribed regions included intergenic transcripts predicted as coding or non-coding as well as those that were antisense to coding sequences. In total, mutation of rpfF, rpfG and rpfC led to alteration in transcript levels (more than fourfold) of approximately 480 genes. The regulatory influence of RpfF and RpfC demonstrated considerable overlap. Contrary to expectation, the regulatory influence of RpfC and RpfG had limited overlap, indicating complexities of the Rpf signalling system. Importantly, functional analysis revealed over 160 new virulence factors within the group of Rpf-regulated genes.
    Original languageEnglish
    Pages (from-to)1058-1069
    Number of pages12
    JournalMolecular Microbiology
    Volume88
    Issue number6
    Early online date26 Apr 2013
    DOIs
    Publication statusPublished - Jun 2013

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    Corrigendum: High-resolution transcriptional analysis of the regulatory influence of cell-to-cell signalling reveals novel genes that contribute to Xanthomonas phytopathogenesis

    An, S-Q., Febrer, M., McCarthy, Y., Tang, D-J., Clissold, L., Kaithakottil, G., Swarbreck, D., Tang, J-L., Rogers, J., Dow, J. M. & Ryan, R. P., May 2017, In : Molecular Microbiology. 104, 4, p. 693-694 2 p.

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    An, S-Q., Febrer, M., McCarthy, Y., Tang, D-J., Clissold, L., Kaithakottil, G., Swarbreck, D., Tang, J-L., Rogers, J., Dow, J. M., & Ryan, R. P. (2013). High-resolution transcriptional analysis of the regulatory influence of cell-to-cell signalling reveals novel genes that contribute to Xanthomonas phytopathogenesis. Molecular Microbiology, 88(6), 1058-1069. https://doi.org/10.1111/mmi.12229