High-throughput root phenotyping screens identify genetic loci associated with root architectural traits in Brassica napus under contrasting phosphate availabilities

Lei Shi; Taoxiong Shi; Martin R. Broadley; Philip J. White; Yan Long; Jinling Meng; Fangsen Xu; John P. Hammond

doi:10.1093/aob/mcs245

High-throughput root phenotyping screens identify genetic loci associated with root architectural traits in Brassica napus under contrasting phosphate availabilities

Lei Shi, Taoxiong Shi, Martin R. Broadley, Philip J. White, Yan Long, Jinling Meng, Fangsen Xu, John P. Hammond

Research output: Contribution to journal › Article › peer-review

92 Citations (Scopus)

Abstract

Phosphate (Pi) deciency in soils is a major limiting factor for crop growth worldwide. Plant growth under low Pi conditions correlates with root architectural traits and it may therefore be possible to select these traits for crop improvement. The aim of this study was to characterize root architectural traits, and to test quantitative trait loci (QTL) associated with these traits, under low Pi (LP) and high Pi (HP) availability in Brassica napus.

Root architectural traits were characterized in seedlings of a double haploid (DH) mapping population (n 190) of B. napus [Tapidor Ningyou 7 (TNDH)] using high-throughput phenotyping methods. Primary root length (PRL), lateral root length (LRL), lateral root number (LRN), lateral root density (LRD) and biomass traits were measured 12 d post-germination in agar at LP and HP.

In general, root and biomass traits were highly correlated under LP and HP conditions. Ningyou 7 had greater LRL, LRN and LRD than Tapidor, at both LP and HP availability, but smaller PRL. A cluster of highly significant QTL for LRN, LRD and biomass traits at LP availability were identified on chromosome A03; QTL for PRL were identified on chromosomes A07 and C06.

High-throughput phenotyping of Brassica can be used to identify root architectural traits which correlate with shoot biomass. It is feasible that these traits could be used in crop improvement strategies. The identification of QTL linked to root traits under LP and HP conditions provides further insights on the genetic basis of plant tolerance to P deciency, and these QTL warrant further dissection.

Original language	English
Pages (from-to)	381-389
Number of pages	9
Journal	Annals of Botany
Volume	112
Issue number	2
DOIs	https://doi.org/10.1093/aob/mcs245
Publication status	Published - 2013

Keywords

root
biomass
QTL
oilseed rape
phosphorus
PHOSPHORUS-USE EFFICIENCY
SOIL
QTL ANALYSIS
OILSEED RAPE
STARVATION
Phosphate
genetic
GROWTH-RESPONSE
AGRICULTURE
Brassica napus
SYSTEM ARCHITECTURE
heritability
NATURAL VARIATION
ARABIDOPSIS-THALIANA

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10.1093/aob/mcs245

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@article{c504ad44cea341e5adba4c989a481ca6,

title = "High-throughput root phenotyping screens identify genetic loci associated with root architectural traits in Brassica napus under contrasting phosphate availabilities",

abstract = "Phosphate (Pi) deciency in soils is a major limiting factor for crop growth worldwide. Plant growth under low Pi conditions correlates with root architectural traits and it may therefore be possible to select these traits for crop improvement. The aim of this study was to characterize root architectural traits, and to test quantitative trait loci (QTL) associated with these traits, under low Pi (LP) and high Pi (HP) availability in Brassica napus.Root architectural traits were characterized in seedlings of a double haploid (DH) mapping population (n 190) of B. napus [Tapidor Ningyou 7 (TNDH)] using high-throughput phenotyping methods. Primary root length (PRL), lateral root length (LRL), lateral root number (LRN), lateral root density (LRD) and biomass traits were measured 12 d post-germination in agar at LP and HP.In general, root and biomass traits were highly correlated under LP and HP conditions. Ningyou 7 had greater LRL, LRN and LRD than Tapidor, at both LP and HP availability, but smaller PRL. A cluster of highly significant QTL for LRN, LRD and biomass traits at LP availability were identified on chromosome A03; QTL for PRL were identified on chromosomes A07 and C06.High-throughput phenotyping of Brassica can be used to identify root architectural traits which correlate with shoot biomass. It is feasible that these traits could be used in crop improvement strategies. The identification of QTL linked to root traits under LP and HP conditions provides further insights on the genetic basis of plant tolerance to P deciency, and these QTL warrant further dissection.",

keywords = "root, biomass, QTL, oilseed rape, phosphorus, PHOSPHORUS-USE EFFICIENCY, SOIL, QTL ANALYSIS, OILSEED RAPE, STARVATION, Phosphate, genetic, GROWTH-RESPONSE, AGRICULTURE, Brassica napus, SYSTEM ARCHITECTURE, heritability, NATURAL VARIATION, ARABIDOPSIS-THALIANA",

author = "Lei Shi and Taoxiong Shi and Broadley, {Martin R.} and White, {Philip J.} and Yan Long and Jinling Meng and Fangsen Xu and Hammond, {John P.}",

year = "2013",

doi = "10.1093/aob/mcs245",

language = "English",

volume = "112",

pages = "381--389",

journal = "Annals of Botany",

issn = "0305-7364",

publisher = "Oxford University Press",

number = "2",

}

TY - JOUR

T1 - High-throughput root phenotyping screens identify genetic loci associated with root architectural traits in Brassica napus under contrasting phosphate availabilities

AU - Shi, Lei

AU - Shi, Taoxiong

AU - Broadley, Martin R.

AU - White, Philip J.

AU - Long, Yan

AU - Meng, Jinling

AU - Xu, Fangsen

AU - Hammond, John P.

PY - 2013

Y1 - 2013

N2 - Phosphate (Pi) deciency in soils is a major limiting factor for crop growth worldwide. Plant growth under low Pi conditions correlates with root architectural traits and it may therefore be possible to select these traits for crop improvement. The aim of this study was to characterize root architectural traits, and to test quantitative trait loci (QTL) associated with these traits, under low Pi (LP) and high Pi (HP) availability in Brassica napus.Root architectural traits were characterized in seedlings of a double haploid (DH) mapping population (n 190) of B. napus [Tapidor Ningyou 7 (TNDH)] using high-throughput phenotyping methods. Primary root length (PRL), lateral root length (LRL), lateral root number (LRN), lateral root density (LRD) and biomass traits were measured 12 d post-germination in agar at LP and HP.In general, root and biomass traits were highly correlated under LP and HP conditions. Ningyou 7 had greater LRL, LRN and LRD than Tapidor, at both LP and HP availability, but smaller PRL. A cluster of highly significant QTL for LRN, LRD and biomass traits at LP availability were identified on chromosome A03; QTL for PRL were identified on chromosomes A07 and C06.High-throughput phenotyping of Brassica can be used to identify root architectural traits which correlate with shoot biomass. It is feasible that these traits could be used in crop improvement strategies. The identification of QTL linked to root traits under LP and HP conditions provides further insights on the genetic basis of plant tolerance to P deciency, and these QTL warrant further dissection.

AB - Phosphate (Pi) deciency in soils is a major limiting factor for crop growth worldwide. Plant growth under low Pi conditions correlates with root architectural traits and it may therefore be possible to select these traits for crop improvement. The aim of this study was to characterize root architectural traits, and to test quantitative trait loci (QTL) associated with these traits, under low Pi (LP) and high Pi (HP) availability in Brassica napus.Root architectural traits were characterized in seedlings of a double haploid (DH) mapping population (n 190) of B. napus [Tapidor Ningyou 7 (TNDH)] using high-throughput phenotyping methods. Primary root length (PRL), lateral root length (LRL), lateral root number (LRN), lateral root density (LRD) and biomass traits were measured 12 d post-germination in agar at LP and HP.In general, root and biomass traits were highly correlated under LP and HP conditions. Ningyou 7 had greater LRL, LRN and LRD than Tapidor, at both LP and HP availability, but smaller PRL. A cluster of highly significant QTL for LRN, LRD and biomass traits at LP availability were identified on chromosome A03; QTL for PRL were identified on chromosomes A07 and C06.High-throughput phenotyping of Brassica can be used to identify root architectural traits which correlate with shoot biomass. It is feasible that these traits could be used in crop improvement strategies. The identification of QTL linked to root traits under LP and HP conditions provides further insights on the genetic basis of plant tolerance to P deciency, and these QTL warrant further dissection.

KW - root

KW - biomass

KW - QTL

KW - oilseed rape

KW - phosphorus

KW - PHOSPHORUS-USE EFFICIENCY

KW - SOIL

KW - QTL ANALYSIS

KW - OILSEED RAPE

KW - STARVATION

KW - Phosphate

KW - genetic

KW - GROWTH-RESPONSE

KW - AGRICULTURE

KW - Brassica napus

KW - SYSTEM ARCHITECTURE

KW - heritability

KW - NATURAL VARIATION

KW - ARABIDOPSIS-THALIANA

U2 - 10.1093/aob/mcs245

DO - 10.1093/aob/mcs245

M3 - Article

C2 - 23172414

SN - 0305-7364

VL - 112

SP - 381

EP - 389

JO - Annals of Botany

JF - Annals of Botany

IS - 2

ER -

High-throughput root phenotyping screens identify genetic loci associated with root architectural traits in Brassica napus under contrasting phosphate availabilities

Abstract

Keywords

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