High-throughput root phenotyping screens identify genetic loci associated with root architectural traits in Brassica napus under contrasting phosphate availabilities

Lei Shi, Taoxiong Shi, Martin R. Broadley, Philip J. White, Yan Long, Jinling Meng, Fangsen Xu, John P. Hammond

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    Abstract

    Phosphate (Pi) deciency in soils is a major limiting factor for crop growth worldwide. Plant growth under low Pi conditions correlates with root architectural traits and it may therefore be possible to select these traits for crop improvement. The aim of this study was to characterize root architectural traits, and to test quantitative trait loci (QTL) associated with these traits, under low Pi (LP) and high Pi (HP) availability in Brassica napus.

    Root architectural traits were characterized in seedlings of a double haploid (DH) mapping population (n 190) of B. napus [Tapidor Ningyou 7 (TNDH)] using high-throughput phenotyping methods. Primary root length (PRL), lateral root length (LRL), lateral root number (LRN), lateral root density (LRD) and biomass traits were measured 12 d post-germination in agar at LP and HP.

    In general, root and biomass traits were highly correlated under LP and HP conditions. Ningyou 7 had greater LRL, LRN and LRD than Tapidor, at both LP and HP availability, but smaller PRL. A cluster of highly significant QTL for LRN, LRD and biomass traits at LP availability were identified on chromosome A03; QTL for PRL were identified on chromosomes A07 and C06.

    High-throughput phenotyping of Brassica can be used to identify root architectural traits which correlate with shoot biomass. It is feasible that these traits could be used in crop improvement strategies. The identification of QTL linked to root traits under LP and HP conditions provides further insights on the genetic basis of plant tolerance to P deciency, and these QTL warrant further dissection.

    Original languageEnglish
    Pages (from-to)381-389
    Number of pages9
    JournalAnnals of Botany
    Volume112
    Issue number2
    DOIs
    Publication statusPublished - 2013

    Keywords

    • root
    • biomass
    • QTL
    • oilseed rape
    • phosphorus
    • PHOSPHORUS-USE EFFICIENCY
    • SOIL
    • QTL ANALYSIS
    • OILSEED RAPE
    • STARVATION
    • Phosphate
    • genetic
    • GROWTH-RESPONSE
    • AGRICULTURE
    • Brassica napus
    • SYSTEM ARCHITECTURE
    • heritability
    • NATURAL VARIATION
    • ARABIDOPSIS-THALIANA

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