Histone H2A-H2B binding by Pol α in the eukaryotic replisome contributes to the maintenance of repressive chromatin

Cecile Evrin, Joseph Maman, Aurora Diamante, Luca Pellegrini, Karim Labib (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

The eukaryotic replisome disassembles parental chromatin at DNA replication forks, but then plays a poorly understood role in the re-deposition of the displaced histone complexes onto nascent DNA. Here, we show that yeast DNA polymerase α contains a histone-binding motif that is conserved in human Pol α and is specific for histones H2A and H2B. Mutation of this motif in budding yeast cells does not affect DNA synthesis, but instead abrogates gene silencing at telomeres and mating-type loci. Similar phenotypes are produced not only by mutations that displace Pol α from the replisome, but also by mutation of the previously identified histone-binding motif in the CMG helicase subunit Mcm2, the human orthologue of which was shown to bind to histones H3 and H4. We show that chromatin-derived histone complexes can be bound simultaneously by Mcm2, Pol α and the histone chaperone FACT that is also a replisome component. These findings indicate that replisome assembly unites multiple histone-binding activities, which jointly process parental histones to help preserve silent chromatin during the process of chromosome duplication.

Original languageEnglish
Article numbere99021
Pages (from-to)1-16
Number of pages16
JournalEMBO Journal
Volume37
Issue number19
Early online date13 Aug 2018
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Histones
Chromatin
Maintenance
Yeast
Mutation
DNA
Histone Chaperones
Chromosome Duplication
Saccharomycetales
Telomere
Gene Silencing
DNA-Directed DNA Polymerase
Chromosomes
DNA Replication
Genes
Yeasts
Cells
Phenotype

Keywords

  • DNA polymerase alpha
  • DNA replication
  • histone chaperone
  • histones
  • replisome

Cite this

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abstract = "The eukaryotic replisome disassembles parental chromatin at DNA replication forks, but then plays a poorly understood role in the re-deposition of the displaced histone complexes onto nascent DNA. Here, we show that yeast DNA polymerase α contains a histone-binding motif that is conserved in human Pol α and is specific for histones H2A and H2B. Mutation of this motif in budding yeast cells does not affect DNA synthesis, but instead abrogates gene silencing at telomeres and mating-type loci. Similar phenotypes are produced not only by mutations that displace Pol α from the replisome, but also by mutation of the previously identified histone-binding motif in the CMG helicase subunit Mcm2, the human orthologue of which was shown to bind to histones H3 and H4. We show that chromatin-derived histone complexes can be bound simultaneously by Mcm2, Pol α and the histone chaperone FACT that is also a replisome component. These findings indicate that replisome assembly unites multiple histone-binding activities, which jointly process parental histones to help preserve silent chromatin during the process of chromosome duplication.",
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Histone H2A-H2B binding by Pol α in the eukaryotic replisome contributes to the maintenance of repressive chromatin. / Evrin, Cecile; Maman, Joseph; Diamante, Aurora; Pellegrini, Luca; Labib, Karim (Lead / Corresponding author).

In: EMBO Journal, Vol. 37, No. 19, e99021, 01.10.2018, p. 1-16.

Research output: Contribution to journalArticle

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AU - Labib, Karim

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