Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities

Michael Bruno, Andrew Flaus, Chris Stockdale, Chantal Rencurel, Helder Ferreira, Tom Owen-Hughes

    Research output: Contribution to journalArticle

    149 Citations (Scopus)

    Abstract

    ATP-dependent chromatin remodeling activities function to manipulate chromatin structure during gene regulation. One of the ways in which they do this is by altering the positions of nucleosomes along DNA. Here we provide support for the ability of these complexes to move nucleosomes into positions in which DNA is unraveled from one edge. This is expected to result in the loss of histone-DNA contacts that are important for retention of one H2A/H2B dimer within the nucleosome. Consistent with this we find that several chromatin remodeling complexes are capable of catalyzing the exchange of H2A/H2B dimers between chromatin fragments in an ATP-dependent reaction. This provides eukaryotes with additional means by which they may manipulate chromatin structure.
    Original languageEnglish
    Pages (from-to)1599-1606
    Number of pages8
    JournalMolecular Cell
    Volume12
    Issue number6
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    Chromatin Assembly and Disassembly
    Nucleosomes
    Histones
    Chromatin
    Adenosine Triphosphate
    DNA
    Eukaryota
    Genes

    Keywords

    • Chromatin
    • Nucleosomes

    Cite this

    Bruno, Michael ; Flaus, Andrew ; Stockdale, Chris ; Rencurel, Chantal ; Ferreira, Helder ; Owen-Hughes, Tom. / Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities. In: Molecular Cell. 2003 ; Vol. 12, No. 6. pp. 1599-1606.
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    Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities. / Bruno, Michael; Flaus, Andrew; Stockdale, Chris; Rencurel, Chantal; Ferreira, Helder; Owen-Hughes, Tom.

    In: Molecular Cell, Vol. 12, No. 6, 2003, p. 1599-1606.

    Research output: Contribution to journalArticle

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    AU - Flaus, Andrew

    AU - Stockdale, Chris

    AU - Rencurel, Chantal

    AU - Ferreira, Helder

    AU - Owen-Hughes, Tom

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