hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing

TY - JOUR

T1 - hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing

AU - Leonard, Deana

AU - Ajuh, Paul M.

AU - Lamond, Angus I.

AU - Legerski, Randy J.

PY - 2003/9/5

Y1 - 2003/9/5

N2 - hLodestar/HuF2 belongs to the SNF2 family of proteins. This family of proteins has been shown to play a critical role in altering protein-DNA interactions in a variety of cellular contexts. We have identified an unexpected interaction between hLodestar/HuF2 and CDC5L in both the yeast two-hybrid system and HeLa nuclear extract. CDC5L is a well-characterized pre-mRNA splicing factor in yeast and humans. Our findings demonstrate that hLodestar/HuF2 associates with human splicing complexes. We also found that a truncated hLodestar/HuF2 polypeptide that overlaps with the CDC5L-binding region can inhibit pre-mRNA splicing by disrupting spliceosome assembly. These findings indicate that hLodestar/HuF2 may have a role in pre-mRNA splicing. These data are consistent with a close co-ordination of the transcription and splicing pathways in eukaryotes. Although many members of the DExH/D helicase superfamily have been linked to pre-mRNA splicing, this is the first SNF2 family member to be implicated in this pathway.

AB - hLodestar/HuF2 belongs to the SNF2 family of proteins. This family of proteins has been shown to play a critical role in altering protein-DNA interactions in a variety of cellular contexts. We have identified an unexpected interaction between hLodestar/HuF2 and CDC5L in both the yeast two-hybrid system and HeLa nuclear extract. CDC5L is a well-characterized pre-mRNA splicing factor in yeast and humans. Our findings demonstrate that hLodestar/HuF2 associates with human splicing complexes. We also found that a truncated hLodestar/HuF2 polypeptide that overlaps with the CDC5L-binding region can inhibit pre-mRNA splicing by disrupting spliceosome assembly. These findings indicate that hLodestar/HuF2 may have a role in pre-mRNA splicing. These data are consistent with a close co-ordination of the transcription and splicing pathways in eukaryotes. Although many members of the DExH/D helicase superfamily have been linked to pre-mRNA splicing, this is the first SNF2 family member to be implicated in this pathway.

KW - CDC5L

KW - hLodestar/HuF2

KW - Pre-mRNA splicing

KW - SNF2

KW - Spliceosome

UR - https://www.scopus.com/pages/publications/0043162024

U2 - 10.1016/S0006-291X(03)01486-4

DO - 10.1016/S0006-291X(03)01486-4

M3 - Article

C2 - 12927788

AN - SCOPUS:0043162024

SN - 0006-291X

VL - 308

SP - 793

EP - 801

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -