TY - JOUR
T1 - Homology of AFLP products in three mapping populations of barley
AU - Waugh, R.
AU - Bonar, N.
AU - Baird, E.
AU - Thomas, B.
AU - Graner, A.
AU - Hayes, P.
AU - Powell, W.
PY - 1997/7
Y1 - 1997/7
N2 - Segregation of 850 polymorphic AFLP (amplified fragment length polymorphism) fragments was followed in three different doubled haploid (DH) barley populations, Dicktoo x Morex (DM), Igri x Franka (IF) and Blenheim x E224/3 (BE), which had previously been used to construct linkage maps using other molecular markers. The final maps consisted of 310, 655 and 474 markers, of which 234, 194 and 376, respectively, were AFLPs. A comparison of profiles from the parental lines identified 51 similar-sized AFLPs segregating in both DM and IF populations, 20 in the DM and BE populations and 18 in the IF and BE populations. Eight segregated in all three. Analysis of the complete datasets for each of the populations using Joinmap V.2. indicated that in general terms each of the AFLPs which were polymorphic in more than population mapped to the same genetic locus. The number of co-dominant markers segregating in a single population ranged from 6% for DM to 12.6% for IF. These results are discussed in the context of using AFLP in genetic linkage and diversity studies.
AB - Segregation of 850 polymorphic AFLP (amplified fragment length polymorphism) fragments was followed in three different doubled haploid (DH) barley populations, Dicktoo x Morex (DM), Igri x Franka (IF) and Blenheim x E224/3 (BE), which had previously been used to construct linkage maps using other molecular markers. The final maps consisted of 310, 655 and 474 markers, of which 234, 194 and 376, respectively, were AFLPs. A comparison of profiles from the parental lines identified 51 similar-sized AFLPs segregating in both DM and IF populations, 20 in the DM and BE populations and 18 in the IF and BE populations. Eight segregated in all three. Analysis of the complete datasets for each of the populations using Joinmap V.2. indicated that in general terms each of the AFLPs which were polymorphic in more than population mapped to the same genetic locus. The number of co-dominant markers segregating in a single population ranged from 6% for DM to 12.6% for IF. These results are discussed in the context of using AFLP in genetic linkage and diversity studies.
KW - AFLP
KW - Barley
KW - Linkage maps
KW - Product homology
UR - http://www.scopus.com/inward/record.url?scp=0030872311&partnerID=8YFLogxK
U2 - 10.1007/s004380050502
DO - 10.1007/s004380050502
M3 - Article
C2 - 9268022
AN - SCOPUS:0030872311
SN - 0026-8925
VL - 255
SP - 311
EP - 321
JO - Molecular and General Genetics MGG
JF - Molecular and General Genetics MGG
IS - 3
ER -