Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner

Stephan Wawra, Judith Bain, Elaine Durward, Irene de Bruijn, Kirsty L. Minor, Anja Matena, Lars Löbach, Stephen C. Whisson, Peter Bayer, Andrew J. Porter, Paul R. J. Birch, Chris J. Secombes, Pieter van West

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    40 Citations (Scopus)

    Abstract

    The eukaryoticoomycetes, or water molds, contain several species that are devastating pathogens of plants and animals. During infection, oomycetes translocate effector proteins into host cells, where they interfere with host-defense responses. For several oomycete effectors (i.e., the RxLR-effectors) it has been shown that their N-terminal polypeptides are important for the delivery into the host. Here we demonstrate that the putative RxLR-like effector, host-targeting protein 1 (SpHtp1), from the fish pathogen Saprolegnia parasitica translocates specifically inside host cells. We further demonstrate that cell-surface binding and uptake of this effector protein is mediated by an interaction with tyrosine-O-sulfate-modified cell-surface molecules and not via phospholipids, as has been reported for RxLR-effectors from plant pathogenic oomycetes. These results reveal an effector translocation route based on tyrosine-O-sulfate binding, which could be highly relevant for a wide range of host-microbe interactions.

    Original languageEnglish
    Pages (from-to)2096-2101
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume109
    Issue number6
    DOIs
    Publication statusPublished - 7 Feb 2012

    Keywords

    • protein translocation
    • Phytophthora
    • Plasmodium
    • PATHOGEN PHYTOPHTHORA-INFESTANS
    • SOJAE-EFFECTOR AVR1B
    • PLANT-CELLS
    • ERYTHROCYTE
    • SECRETION
    • VIRULENCE
    • BINDING
    • GENOME
    • ENTRY
    • AVIRULENCE

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