How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use?

Nicholas J Schurch, Pietá Schofield, Marek Gierliński, Christian Cole, Alexander Sherstnev, Vijender Singh, Nicola Wrobel, Karim Gharbi, Gordon G Simpson (Lead / Corresponding author), Tom Owen-Hughes (Lead / Corresponding author), Mark Blaxter (Lead / Corresponding author), Geoffrey J Barton (Lead / Corresponding author)