How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use?

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• Finished

  Diversifying Transcription Termination Function

  Barton, G. (Investigator) & Simpson, G. (Investigator)

  Biotechnology and Biological Sciences Research Council

  1/06/15 → 31/05/19

  Project: Research

  • RNA Sequencing 100%
  • Arabidopsis 100%
  • RNA Sequencing (RNA-seq) 86%
  • RNA Sequence 80%
  • Arabidopsis Thaliana 65%

• The Arabidopsis Epitranscriptome (Joint with University of Nottingham)

  Barton, G. (Investigator) & Simpson, G. (Investigator)

  Biotechnology and Biological Sciences Research Council

  1/04/15 → 31/03/19

  Project: Research

  • RNA Sequencing 100%
  • Arabidopsis 87%
  • RNA Sequencing (RNA-seq) 86%
  • RNA Sequence 80%
  • Arabidopsis Thaliana 61%

• Determination of Authentic Substrates for Parkinson's Disease Associated Protein Kinases, LRRK and PINK1 (supplement to Clinical Fellowship)

  Barton, G. (Investigator), Muqit, M. (Investigator) & Simpson, G. (Investigator)

  1/07/12 → 31/12/15

  Project: Research

  • RNA Sequencing 100%
  • RNA Sequencing (RNA-seq) 89%
  • RNA Sequence 84%
  • RNA 63%
  • Arabidopsis Thaliana 56%

• The Non-Coding Arabidopsis Genome

  Barton, G. (Investigator) & Simpson, G. (Investigator)

  1/07/12 → 31/12/15

  Project: Research

  • RNA Sequencing 100%
  • RNA Sequencing (RNA-seq) 89%
  • RNA Sequence 84%
  • RNA 63%
  • Arabidopsis Thaliana 56%

• Mechanisms for Remodelling Chromatin (Senior Fellowship Renewal)

  Owen-Hughes, T. (Investigator)

  1/01/12 → 31/05/19

  Project: Research

  • Nucleosome 100%
  • Histone 51%
  • Enzyme 43%
  • Chromatin Remodeling 43%
  • Chromatin 42%

• Aref#d: 21678. Elucidating Mechanisms and Roles of Alternative Polyadenylation

  Barton, G. (Investigator) & Simpson, G. (Investigator)

  1/12/09 → 16/05/13

  Project: Research

  • RNA Sequencing 100%
  • RNA Sequencing (RNA-seq) 89%
  • RNA 86%
  • Alternative Polyadenylation 80%
  • Arabidopsis Thaliana 78%