HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens

Antonio Francioso (Lead / Corresponding author), Sergio Fanelli, Daniele Vigli, Laura Ricceri, Rosaria A. Cavallaro, Alessia Baseggio Conrado, Mario Fontana, Maria D'Erme, Luciana Mosca

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    10 Citations (Scopus)
    596 Downloads (Pure)


    There is an increasing interest for analytical methods aimed to detect biological sulfur-containing amines, because of their involvement in human diseases and metabolic disorders. This work describes an improved HPLC method for the determination of sulfur containing amino acids and amines from different biological matrices. We optimized a pre-column derivatization procedure using dabsyl chloride, in which dabsylated products can be monitored spectrophotometrically at 460 nm. This method allows the simultaneous analysis of biogenic amines, amino acids and sulfo-amino compounds including carnosine, dopamine, epinephrine, glutathione, cysteine, taurine, lanthionine, and cystathionine in brain specimens, urines, plasma, and cell lysates. Moreover, the method is suitable for the study of physiological and non-physiological derivatives of taurine and glutathione such as hypotaurine, homotaurine, homocysteic acid and S-acetylglutathione. The present method displays good efficiency of derivatization, having the advantage to give rise to stable products compared to other derivatizing agents such as o-phthalaldehyde and dansyl chloride.With this method, we provide a tool to study sulfur cycle from a metabolic point of view in relation to the pattern of biological amino-compounds, allowing researchers to get a complete scenario of organic sulfur and amino metabolism in tissues and cells.

    Original languageEnglish
    Title of host publicationTaurine 10
    EditorsDong-Hee Lee, Stephen W. Schaffer, Eunkyue Park, Ha Won Kim
    Place of PublicationDordrecht
    Number of pages15
    ISBN (Electronic)9789402410792
    ISBN (Print)9789402410778
    Publication statusPublished - 2017
    Event20th International Taurine Meeting: “Taurine and Brain Health” - Plaza Hotel, Seoul, Korea, Republic of
    Duration: 23 May 201627 May 2016

    Publication series

    NameAdvances in Experimental Medicine and Biology
    ISSN (Print)0065-2598
    ISSN (Electronic)2214-8019


    Conference20th International Taurine Meeting
    Abbreviated titleINTAM20
    Country/TerritoryKorea, Republic of
    Internet address


    • High pressure/performance liquid chromatography
    • Sulfur-containing bioactive compounds
    • Dabsyl chloride


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