Research output per year
Research output per year
Antonio Francioso (Lead / Corresponding author), Sergio Fanelli, Daniele Vigli, Laura Ricceri, Rosaria A. Cavallaro, Alessia Baseggio Conrado, Mario Fontana, Maria D'Erme, Luciana Mosca
Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
There is an increasing interest for analytical methods aimed to detect biological sulfur-containing amines, because of their involvement in human diseases and metabolic disorders. This work describes an improved HPLC method for the determination of sulfur containing amino acids and amines from different biological matrices. We optimized a pre-column derivatization procedure using dabsyl chloride, in which dabsylated products can be monitored spectrophotometrically at 460 nm. This method allows the simultaneous analysis of biogenic amines, amino acids and sulfo-amino compounds including carnosine, dopamine, epinephrine, glutathione, cysteine, taurine, lanthionine, and cystathionine in brain specimens, urines, plasma, and cell lysates. Moreover, the method is suitable for the study of physiological and non-physiological derivatives of taurine and glutathione such as hypotaurine, homotaurine, homocysteic acid and S-acetylglutathione. The present method displays good efficiency of derivatization, having the advantage to give rise to stable products compared to other derivatizing agents such as o-phthalaldehyde and dansyl chloride.With this method, we provide a tool to study sulfur cycle from a metabolic point of view in relation to the pattern of biological amino-compounds, allowing researchers to get a complete scenario of organic sulfur and amino metabolism in tissues and cells.
Original language | English |
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Title of host publication | Taurine 10 |
Editors | Dong-Hee Lee, Stephen W. Schaffer, Eunkyue Park, Ha Won Kim |
Place of Publication | Dordrecht |
Publisher | Springer |
Pages | 535-549 |
Number of pages | 15 |
ISBN (Electronic) | 9789402410792 |
ISBN (Print) | 9789402410778 |
DOIs | |
Publication status | Published - 2017 |
Event | 20th International Taurine Meeting: “Taurine and Brain Health” - Plaza Hotel, Seoul, Korea, Republic of Duration: 23 May 2016 → 27 May 2016 http://taurine2016.org/register/2016_met/main.html |
Name | Advances in Experimental Medicine and Biology |
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Publisher | Springer |
Volume | 975 |
ISSN (Print) | 0065-2598 |
ISSN (Electronic) | 2214-8019 |
Conference | 20th International Taurine Meeting |
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Abbreviated title | INTAM20 |
Country/Territory | Korea, Republic of |
City | Seoul |
Period | 23/05/16 → 27/05/16 |
Internet address |
Research output: Chapter in Book/Report/Conference proceeding › Conference contribution