HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens

Antonio Francioso; Sergio Fanelli; Daniele Vigli; Laura Ricceri; Rosaria A. Cavallaro; Alessia Baseggio Conrado; Mario Fontana; Maria D'Erme; Luciana Mosca

doi:10.1007/978-94-024-1079-2_42

HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens

Antonio Francioso (Lead / Corresponding author)
, Sergio Fanelli
, Daniele Vigli
, Laura Ricceri
, Rosaria A. Cavallaro
, Alessia Baseggio Conrado
, Mario Fontana
, Maria D'Erme
, Luciana Mosca

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

12 Citations (Scopus)

871 Downloads (Pure)

Abstract

There is an increasing interest for analytical methods aimed to detect biological sulfur-containing amines, because of their involvement in human diseases and metabolic disorders. This work describes an improved HPLC method for the determination of sulfur containing amino acids and amines from different biological matrices. We optimized a pre-column derivatization procedure using dabsyl chloride, in which dabsylated products can be monitored spectrophotometrically at 460 nm. This method allows the simultaneous analysis of biogenic amines, amino acids and sulfo-amino compounds including carnosine, dopamine, epinephrine, glutathione, cysteine, taurine, lanthionine, and cystathionine in brain specimens, urines, plasma, and cell lysates. Moreover, the method is suitable for the study of physiological and non-physiological derivatives of taurine and glutathione such as hypotaurine, homotaurine, homocysteic acid and S-acetylglutathione. The present method displays good efficiency of derivatization, having the advantage to give rise to stable products compared to other derivatizing agents such as o-phthalaldehyde and dansyl chloride.With this method, we provide a tool to study sulfur cycle from a metabolic point of view in relation to the pattern of biological amino-compounds, allowing researchers to get a complete scenario of organic sulfur and amino metabolism in tissues and cells.

Original language	English
Title of host publication	Taurine 10
Editors	Dong-Hee Lee, Stephen W. Schaffer, Eunkyue Park, Ha Won Kim
Place of Publication	Dordrecht
Publisher	Springer
Pages	535-549
Number of pages	15
ISBN (Electronic)	9789402410792
ISBN (Print)	9789402410778
DOIs	https://doi.org/10.1007/978-94-024-1079-2_42
Publication status	Published - 2017
Event	20th International Taurine Meeting: “Taurine and Brain Health” - Plaza Hotel, Seoul, Korea, Republic of Duration: 23 May 2016 → 27 May 2016 http://taurine2016.org/register/2016_met/main.html

Publication series

Name	Advances in Experimental Medicine and Biology
Publisher	Springer
Volume	975
ISSN (Print)	0065-2598
ISSN (Electronic)	2214-8019

Conference

Conference	20th International Taurine Meeting
Abbreviated title	INTAM20
Country/Territory	Korea, Republic of
City	Seoul
Period	23/05/16 → 27/05/16
Internet address	http://taurine2016.org/register/2016_met/main.html

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

High pressure/performance liquid chromatography
Sulfur-containing bioactive compounds
Dabsyl chloride

Access to Document

10.1007/978-94-024-1079-2_42

Author Accepted Manuscript
The final publication is available at Springer via http://dx.doi.org/10.1007/978-94-024-1079-2_42.
Accepted author manuscript, 941 KB

Erratum to: HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens
Francioso, A., Fanelli, S., Vigli, D., Ricceri, L., Cavallaro, R. A., Conrado, A. B., Fontana, M., D'Erme, M. & Mosca, L., 2017, Taurine 10. Lee, D.-H., Schaffer, S. W., Park, E. & Kim, H. W. (eds.). Dordrecht: Springer , p. E1 1 p. (Advances in Experimental Medicine and Biology; vol. 975).
Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Link opens in a new tab Citation (Scopus)

Cite this

Francioso, A., Fanelli, S., Vigli, D., Ricceri, L., Cavallaro, R. A., Conrado, A. B., Fontana, M., D'Erme, M., & Mosca, L. (2017). HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens. In D.-H. Lee, S. W. Schaffer, E. Park, & H. W. Kim (Eds.), Taurine 10 (pp. 535-549). (Advances in Experimental Medicine and Biology; Vol. 975). Springer . https://doi.org/10.1007/978-94-024-1079-2_42

@inproceedings{a18eddb1331043c7979ffbdb26f79d39,

title = "HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens",

abstract = "There is an increasing interest for analytical methods aimed to detect biological sulfur-containing amines, because of their involvement in human diseases and metabolic disorders. This work describes an improved HPLC method for the determination of sulfur containing amino acids and amines from different biological matrices. We optimized a pre-column derivatization procedure using dabsyl chloride, in which dabsylated products can be monitored spectrophotometrically at 460 nm. This method allows the simultaneous analysis of biogenic amines, amino acids and sulfo-amino compounds including carnosine, dopamine, epinephrine, glutathione, cysteine, taurine, lanthionine, and cystathionine in brain specimens, urines, plasma, and cell lysates. Moreover, the method is suitable for the study of physiological and non-physiological derivatives of taurine and glutathione such as hypotaurine, homotaurine, homocysteic acid and S-acetylglutathione. The present method displays good efficiency of derivatization, having the advantage to give rise to stable products compared to other derivatizing agents such as o-phthalaldehyde and dansyl chloride.With this method, we provide a tool to study sulfur cycle from a metabolic point of view in relation to the pattern of biological amino-compounds, allowing researchers to get a complete scenario of organic sulfur and amino metabolism in tissues and cells.",

keywords = "High pressure/performance liquid chromatography , Sulfur-containing bioactive compounds , Dabsyl chloride ",

author = "Antonio Francioso and Sergio Fanelli and Daniele Vigli and Laura Ricceri and Cavallaro, \{Rosaria A.\} and Conrado, \{Alessia Baseggio\} and Mario Fontana and Maria D'Erme and Luciana Mosca",

note = "This work has been supported by FILAS project (Prot.Filas\_RU.2014.1020) and by GNOSIS S.p.A.; 20th International Taurine Meeting : “Taurine and Brain Health” , INTAM20 ; Conference date: 23-05-2016 Through 27-05-2016",

year = "2017",

doi = "10.1007/978-94-024-1079-2\_42",

language = "English",

isbn = "9789402410778",

series = "Advances in Experimental Medicine and Biology",

publisher = "Springer ",

pages = "535--549",

editor = "Lee, \{Dong-Hee \} and Schaffer, \{Stephen W. \} and Park, \{Eunkyue \} and Kim, \{Ha Won \}",

booktitle = "Taurine 10",

url = "http://taurine2016.org/register/2016\_met/main.html",

}

Francioso, A, Fanelli, S, Vigli, D, Ricceri, L, Cavallaro, RA, Conrado, AB, Fontana, M, D'Erme, M & Mosca, L 2017, HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens. in D-H Lee, SW Schaffer, E Park & HW Kim (eds), Taurine 10. Advances in Experimental Medicine and Biology, vol. 975, Springer , Dordrecht, pp. 535-549, 20th International Taurine Meeting, Seoul, Korea, Republic of, 23/05/16. https://doi.org/10.1007/978-94-024-1079-2_42

HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens. / Francioso, Antonio (Lead / Corresponding author); Fanelli, Sergio; Vigli, Daniele et al.
Taurine 10. ed. / Dong-Hee Lee; Stephen W. Schaffer; Eunkyue Park; Ha Won Kim. Dordrecht: Springer , 2017. p. 535-549 (Advances in Experimental Medicine and Biology; Vol. 975).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens

AU - Francioso, Antonio

AU - Fanelli, Sergio

AU - Vigli, Daniele

AU - Ricceri, Laura

AU - Cavallaro, Rosaria A.

AU - Conrado, Alessia Baseggio

AU - Fontana, Mario

AU - D'Erme, Maria

AU - Mosca, Luciana

N1 - This work has been supported by FILAS project (Prot.Filas_RU.2014.1020) and by GNOSIS S.p.A.

PY - 2017

Y1 - 2017

N2 - There is an increasing interest for analytical methods aimed to detect biological sulfur-containing amines, because of their involvement in human diseases and metabolic disorders. This work describes an improved HPLC method for the determination of sulfur containing amino acids and amines from different biological matrices. We optimized a pre-column derivatization procedure using dabsyl chloride, in which dabsylated products can be monitored spectrophotometrically at 460 nm. This method allows the simultaneous analysis of biogenic amines, amino acids and sulfo-amino compounds including carnosine, dopamine, epinephrine, glutathione, cysteine, taurine, lanthionine, and cystathionine in brain specimens, urines, plasma, and cell lysates. Moreover, the method is suitable for the study of physiological and non-physiological derivatives of taurine and glutathione such as hypotaurine, homotaurine, homocysteic acid and S-acetylglutathione. The present method displays good efficiency of derivatization, having the advantage to give rise to stable products compared to other derivatizing agents such as o-phthalaldehyde and dansyl chloride.With this method, we provide a tool to study sulfur cycle from a metabolic point of view in relation to the pattern of biological amino-compounds, allowing researchers to get a complete scenario of organic sulfur and amino metabolism in tissues and cells.

AB - There is an increasing interest for analytical methods aimed to detect biological sulfur-containing amines, because of their involvement in human diseases and metabolic disorders. This work describes an improved HPLC method for the determination of sulfur containing amino acids and amines from different biological matrices. We optimized a pre-column derivatization procedure using dabsyl chloride, in which dabsylated products can be monitored spectrophotometrically at 460 nm. This method allows the simultaneous analysis of biogenic amines, amino acids and sulfo-amino compounds including carnosine, dopamine, epinephrine, glutathione, cysteine, taurine, lanthionine, and cystathionine in brain specimens, urines, plasma, and cell lysates. Moreover, the method is suitable for the study of physiological and non-physiological derivatives of taurine and glutathione such as hypotaurine, homotaurine, homocysteic acid and S-acetylglutathione. The present method displays good efficiency of derivatization, having the advantage to give rise to stable products compared to other derivatizing agents such as o-phthalaldehyde and dansyl chloride.With this method, we provide a tool to study sulfur cycle from a metabolic point of view in relation to the pattern of biological amino-compounds, allowing researchers to get a complete scenario of organic sulfur and amino metabolism in tissues and cells.

KW - High pressure/performance liquid chromatography

KW - Sulfur-containing bioactive compounds

KW - Dabsyl chloride

U2 - 10.1007/978-94-024-1079-2_42

DO - 10.1007/978-94-024-1079-2_42

M3 - Conference contribution

C2 - 28849480

SN - 9789402410778

T3 - Advances in Experimental Medicine and Biology

SP - 535

EP - 549

BT - Taurine 10

A2 - Lee, Dong-Hee

A2 - Schaffer, Stephen W.

A2 - Park, Eunkyue

A2 - Kim, Ha Won

PB - Springer

CY - Dordrecht

T2 - 20th International Taurine Meeting

Y2 - 23 May 2016 through 27 May 2016

ER -

Francioso A, Fanelli S, Vigli D, Ricceri L, Cavallaro RA, Conrado AB et al. HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens. In Lee DH, Schaffer SW, Park E, Kim HW, editors, Taurine 10. Dordrecht: Springer . 2017. p. 535-549. (Advances in Experimental Medicine and Biology). Epub 2017 Aug 29. doi: 10.1007/978-94-024-1079-2_42

HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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Research output

Erratum to: HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens

Cite this