hRRN3 is essential in the SL1-mediated recruitment of RNA Polymerase I to rRNA gene promoters

Gail Miller, Kostya I. Panov, J. Karsten Friedrich, Laura Trinkle-Mulcahy, Angus I. Lamond, Joost C. B. M. Zomerdijk

    Research output: Contribution to journalArticle

    133 Citations (Scopus)

    Abstract

    A crucial step in transcription is the recruitment of RNA polymerase to promoters. In the transcription of human rRNA genes by RNA Polymerase I (Pol I), transcription factor SL1 has a role as the essential core promoter binding factor. Little is known about the mechanism by which Pol I is recruited. We provide evidence for an essential role for hRRN3, the human homologue of a yeast Pol I transcription factor, in this process. We find that whereas the bulk of human Pol I complexes (Ia) are transcriptionally inactive, hRRN3 defines a distinct subpopulation of Pol I complexes (Iß) that supports specific initiation of transcription. Human RRN3 interacts directly with TAF1110 and TAF163 of promoter-selectivity factor SL1. Blocking this connection prevents recruitment of Pol Iß to the rDNA promoter. Furthermore, hRRN3 can be found in transcriptionally autonomous Pol I holoenzyme complexes. We conclude that hRRN3 functions to recruit initiation-competent Pol I to rRNA gene promoters. The essential role for hRRN3 in linking Pol I to SL1 suggests a mechanism for growth control of Pol I transcription.
    Original languageEnglish
    Pages (from-to)1373-1382
    Number of pages10
    JournalThe EMBO Journal
    Volume20
    Issue number6
    DOIs
    Publication statusPublished - Mar 2001

    Keywords

    • Growth control
    • Holoenzyme
    • Nucleolus
    • rRNA
    • Transcription

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