Abstract
Key components of the miRNA-mediated gene regulation pathway are localized in cytoplasmic processing bodies (P-bodies). Mounting evidence suggests that the presence of microscopic P-bodies are not always required for miRNA-mediated gene regulation. Here we have shown that geldanamycin, a well-characterized HSP90 inhibitor, abolishes P-bodies and significantly reduces Argonaute and GW182 protein levels but does not affect the miRNA level and the efficiency of miRNA-mediated gene repression; however, it significantly impairs siRNA loading and the efficacy of exogenous siRNA. Our data suggests that HSP90 protein chaperones Argonautes before binding RNA and may facilitate efficient loading of small RNA.
Original language | English |
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Pages (from-to) | 1462-1469 |
Number of pages | 8 |
Journal | Molecular Biology of the Cell |
Volume | 21 |
Issue number | 9 |
DOIs | |
Publication status | Published - 1 May 2010 |
Keywords
- MEDIATED TRANSLATIONAL REPRESSION
- MESSENGER-RNA DECAY
- LET-7 MICRORNA
- GW/P-BODIES
- PAZ DOMAIN
- GW182
- MIRNA
- TARGET
- REQUIRES
- BINDING