Human acrocentric chromosomes with transcriptionally silent nucleolar organizer regions associate with nucleoli

Gareth J. Sullivan, Joanna M. Bridger, Andrew P. Cuthbert, Robert F. Newbold, Wendy A. Bickmore, Brian McStay

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    Abstract

    Human ribosomal gene repeats are distributed among five nucleolar organizer regions (NORs) on the p arms of acrocentric chromosomes. On exit from mitosis, nucleoli form around individual active NORs. As cells progress through the cycle, these mini-nucleoli fuse to form large nucleoli incorporating multiple NORs. It is generally assumed that nucleolar incorporation of individual NORs is dependent on ribosomal gene transcription. To test this assumption, we determined the nuclear location of individual human acrocentric chromosomes, and their associated NORs, in mouse> human cell hybrids. Human ribosomal genes are transcriptionally silent in this context. Combined immunofluorescence and in situ hybridization (immuno-FISH) on three-dimensional preserved nuclei showed that human acrocentric chromosomes associate with hybrid cell nucleoli. Analysis of purified nucleoli demonstrated that human and mouse NORs are equally likely to be within a hybrid cell nucleolus. This is supported further by the observation that murine upstream binding factor can associate with human NORs. Incorporation of silent NORs into mature nucleoli raises interesting issues concerning the maintenance of the activity status of individual NORs.
    Original languageEnglish
    Pages (from-to)2867-2877
    Number of pages11
    JournalThe EMBO Journal
    Volume20
    Issue number11
    DOIs
    Publication statusPublished - Jun 2001

    Keywords

    • Human acrocentric chromosomes
    • Nucleolar organiser regions
    • Nucleoli
    • Somatic cell hybrids
    • UBF

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    Sullivan, G. J., Bridger, J. M., Cuthbert, A. P., Newbold, R. F., Bickmore, W. A., & McStay, B. (2001). Human acrocentric chromosomes with transcriptionally silent nucleolar organizer regions associate with nucleoli. The EMBO Journal, 20(11), 2867-2877. https://doi.org/10.1093/emboj/20.11.2867