Projects per year
Abstract
Gene expression in eukaryotes is dependent on the mRNA methyl cap which mediates mRNA processing and translation initiation. Synthesis of the methyl cap initiates with addition of 7-methylguanosine to the initiating nucleotide of RNA pol II transcripts, which occurs predominantly during transcription and in mammals is catalyzed by RNGTT (RNA guanylyltransferase and 5' phosphatase) and RNMT (RNA guanine-7 methyltransferase). RNMT has a methyltransferase domain and an N-terminal domain whose function is unclear; it is conserved in mammals but not required for cap methyltransferase activity. We report that the RNMT N-terminal domain is necessary and sufficient for RNMT recruitment to transcription initiation sites and that recruitment occurs in a DRB-dependent manner. The RNMT-activating subunit, RAM, is also recruited to transcription initiation sites via an interaction with RNMT. The RNMT N-terminal domain is required for transcript expression, translation and cell proliferation.
Original language | English |
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Pages (from-to) | 67-73 |
Number of pages | 7 |
Journal | Biochemical Journal |
Volume | 455 |
Issue number | 1 |
DOIs | |
Publication status | Published - 18 Jul 2013 |
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Dive into the research topics of 'Human cap methyltransferase (RNMT) N-terminal non-catalytic domain mediates recruitment to transcription initiation sites'. Together they form a unique fingerprint.Projects
- 1 Finished
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Aref#d: 19352. The Mechanism of Myc function in cell proliferation and cancer (Career Development Fellowship)
Cowling, V. (Investigator)
3/12/07 → 2/01/14
Project: Research
Student theses
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Cell cycle-dependent regulation of the human RNA cap methyltransferase (RNMT)
Aregger, M. (Author), Cowling, V. (Supervisor), 2013Student thesis: Doctoral Thesis › Doctor of Philosophy
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