Gene expression in eukaryotes is dependent on the mRNA methyl cap which mediates mRNA processing and translation initiation. Synthesis of the methyl cap initiates with addition of 7-methylguanosine to the initiating nucleotide of RNA pol II transcripts, which occurs predominantly during transcription and in mammals is catalyzed by RNGTT (RNA guanylyltransferase and 5' phosphatase) and RNMT (RNA guanine-7 methyltransferase). RNMT has a methyltransferase domain and an N-terminal domain whose function is unclear; it is conserved in mammals but not required for cap methyltransferase activity. We report that the RNMT N-terminal domain is necessary and sufficient for RNMT recruitment to transcription initiation sites and that recruitment occurs in a DRB-dependent manner. The RNMT-activating subunit, RAM, is also recruited to transcription initiation sites via an interaction with RNMT. The RNMT N-terminal domain is required for transcript expression, translation and cell proliferation.
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Supervisor: Cowling, V. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of PhilosophyFile