Human papillomavirus and salivary gland neoplasia: a p16INK4 immunohistochemical and in situ hybridisation study

M. S. Miah (Lead / Corresponding author), S. Majumdar, S. White, M. Robinson, N. Kernohan

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Objective:: This study aimed to evaluate the association between human papillomavirus infection and salivary gland tumours in a Scottish cohort. Methods:: Specimens from a range of salivary gland tumours operated on between 1997 and 2012 were studied. A tissue microarray constructed from tissue blocks was subjected to p16<sup>INK4</sup> (cyclin-dependent kinase inhibitor 2A) immunohistochemistry and in situ hybridisation using probes specific for human papillomavirus, including types 16 and 18. Results:: A total of 61 tumours (benign and malignant) were deemed suitable for the study. p16<sup>INK4</sup> staining yielded three (4.9 per cent) positive samples: one small cell carcinoma, one squamous cell carcinoma and one poorly differentiated carcinoma. Human papillomavirus in situ hybridisation demonstrated a positive signal in the latter sample only (1.6 per cent). Conclusion:: This study demonstrated a very low human papillomavirus detection rate in salivary gland tumours. It can therefore be concluded that human papillomavirus infection is unlikely to play a role in salivary gland neoplasia. Rare human papillomavirus positive cases should be carefully evaluated to exclude the possibility of a metastatic lesion.

Original languageEnglish
Pages (from-to)1000-1003
Number of pages4
JournalJournal of Laryngology and Otology
Issue number10
Early online date20 Jul 2015
Publication statusPublished - Oct 2015


  • Human papillomavirus
  • Neoplasms
  • Salivary glands

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