TY - JOUR
T1 - Human salivary proteome
T2 - a resource of potential biomarkers for oral cancer
AU - Sivadasan, Priya
AU - Gupta, Manoj Kumar
AU - Sathe, Gajanan J.
AU - Balakrishnan, Lavanya
AU - Palit, Priyanka
AU - Gowda, Harsha
AU - Suresh, Amritha
AU - Kuriakose, Moni Abraham
AU - Sirdeshmukh, Ravi
N1 - Funding Information:
We acknowledge the Department of Biotechnology (DBT), Govt. of India ( BT/PR13505/Med/30/276/2010 and BT/01/CEIB/11/IV/05 Dated 22/08/13 ) for financial support. PS is a recipient of Senior Research fellowship from the University Grants Commission, India. MKG is a recipient of Senior Research fellowship from Council of Scientific and Industrial Research (CSIR), India. HG is a WellcomeTrust/DBT India Alliance Early Career Fellow. AS is a recipient of Young Investigator award from DBT. Sneha M. Pinto (IOB) helped in the initial phase of experiments.
Publisher Copyright:
© 2015 Elsevier B.V.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2015/9/8
Y1 - 2015/9/8
N2 - Proteins present in human saliva offer an immense potential for clinical applications. However, exploring salivary proteome is technically challenged due to the presence of amylase and albumin in high abundance. In this study, we used four workflows to analyze human saliva from healthy individuals which involved depletion of abundant proteins using affinity-based separation methods followed by protein or peptide fractionation and high resolution mass spectrometry analysis. We identified a total of 1256 human salivary proteins, 292 of them being reported for the first time. All identifications were verified for any shared proteins/peptides from the salivary microbiome that may conflict with the human protein identifications. On integration of our results with the analyses reported earlier, we arrived at an updated human salivary proteome containing 3449 proteins, 808 of them have been reported as differentially expressed proteins in oral cancer tissues. The secretory nature of 598 of the 808 proteins has also been supported on the basis of the presence of signal sequence, transmembrane domain or association with exosomes. From this subset, we provide a priority list of 139 proteins along with their proteotypic peptides, which may serve as a reference for targeted investigations as secretory markers for clinical applications in oral malignancies. This article is part of a Special Issue entitled: Proteomics in India.
AB - Proteins present in human saliva offer an immense potential for clinical applications. However, exploring salivary proteome is technically challenged due to the presence of amylase and albumin in high abundance. In this study, we used four workflows to analyze human saliva from healthy individuals which involved depletion of abundant proteins using affinity-based separation methods followed by protein or peptide fractionation and high resolution mass spectrometry analysis. We identified a total of 1256 human salivary proteins, 292 of them being reported for the first time. All identifications were verified for any shared proteins/peptides from the salivary microbiome that may conflict with the human protein identifications. On integration of our results with the analyses reported earlier, we arrived at an updated human salivary proteome containing 3449 proteins, 808 of them have been reported as differentially expressed proteins in oral cancer tissues. The secretory nature of 598 of the 808 proteins has also been supported on the basis of the presence of signal sequence, transmembrane domain or association with exosomes. From this subset, we provide a priority list of 139 proteins along with their proteotypic peptides, which may serve as a reference for targeted investigations as secretory markers for clinical applications in oral malignancies. This article is part of a Special Issue entitled: Proteomics in India.
KW - Mass spectrometry
KW - Oral cancer
KW - Proteomics
KW - Saliva
KW - Salivary proteome
UR - http://www.scopus.com/inward/record.url?scp=84943356214&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2015.05.039
DO - 10.1016/j.jprot.2015.05.039
M3 - Article
C2 - 26073025
AN - SCOPUS:84943356214
SN - 1874-3919
VL - 127
SP - 89
EP - 95
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - Part A
ER -