Hydrodynamic stretching for prostate cancer detection

Yuri Belotti; Michael Conneely; Scott Palmer; Tianjun Huang; Paul Andrew Campbell; Stephen McKenna; Ghulam Nabi; David McGloin

doi:10.1117/12.2179201

Hydrodynamic stretching for prostate cancer detection

Yuri Belotti, Michael Conneely, Scott Palmer, Tianjun Huang, Paul Andrew Campbell, Stephen McKenna, Ghulam Nabi, David McGloin

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Advances in diagnostic technologies enabled scientists to link a large number of diseases with structural changes of the intracellular organisation. This intrinsic biophysical characteristic opened up the possibility to perform clinical assessments based on the measurement of single-cell mechanical properties. In this work, we combine microfluidics, high speed imaging and computational automatic tracking to measure the single-cell deformability of large samples of prostate cancer cells at a rate of ∼ 10⁴cells/s. Such a high throughput accounts for the inherent heterogeneity of biological samples and enabled us to extract statistically meaningful signatures from each cell population. In addition, using our technique we investigate the effect of Latrunculin A to the cellular stiffness.

Original language	English
Title of host publication	Bio-MEMS and Medical Microdevices II
Editors	Sander van den Driesche
Place of Publication	Bellingham
Publisher	SPIE-International Society for Optical Engineering
Volume	9518
ISBN (Print)	9781628416411
DOIs	https://doi.org/10.1117/12.2179201
Publication status	Published - 1 Jun 2015
Event	SPIE Microtechnologies 2015: Bio-MEMS and Medical Microdevices II - Hotel Alimara, Barcelona, Spain Duration: 4 May 2015 → 6 May 2015 http://spie.org/conferences-and-exhibitions/past-events/past-conferences-and-exhibitions/past-events/microtechnologies/microtechnologies-2015 (Link to conference)

Publication series

Name	Proceedings of SPIE
Publisher	SPIE
Volume	9518

Conference

Conference	SPIE Microtechnologies 2015: Bio-MEMS and Medical Microdevices II
Country/Territory	Spain
City	Barcelona
Period	4/05/15 → 6/05/15
Internet address	http://spie.org/conferences-and-exhibitions/past-events/past-conferences-and-exhibitions/past-events/microtechnologies/microtechnologies-2015 (Link to conference)

Keywords

Automated Tracking
Cell Mechanics
High Speed Imaging
High Throughput Diagnosis
Microfluidics
Prostate Cancer

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1117/12.2179201

Cite this

Belotti, Y., Conneely, M., Palmer, S., Huang, T., Campbell, P. A., McKenna, S., Nabi, G., & McGloin, D. (2015). Hydrodynamic stretching for prostate cancer detection. In S. van den Driesche (Ed.), Bio-MEMS and Medical Microdevices II (Vol. 9518). Article 95180H (Proceedings of SPIE; Vol. 9518). SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2179201

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title = "Hydrodynamic stretching for prostate cancer detection",

abstract = "Advances in diagnostic technologies enabled scientists to link a large number of diseases with structural changes of the intracellular organisation. This intrinsic biophysical characteristic opened up the possibility to perform clinical assessments based on the measurement of single-cell mechanical properties. In this work, we combine microfluidics, high speed imaging and computational automatic tracking to measure the single-cell deformability of large samples of prostate cancer cells at a rate of ∼ 104cells/s. Such a high throughput accounts for the inherent heterogeneity of biological samples and enabled us to extract statistically meaningful signatures from each cell population. In addition, using our technique we investigate the effect of Latrunculin A to the cellular stiffness.",

keywords = "Automated Tracking, Cell Mechanics, High Speed Imaging, High Throughput Diagnosis, Microfluidics, Prostate Cancer",

author = "Yuri Belotti and Michael Conneely and Scott Palmer and Tianjun Huang and Campbell, {Paul Andrew} and Stephen McKenna and Ghulam Nabi and David McGloin",

year = "2015",

month = jun,

day = "1",

doi = "10.1117/12.2179201",

language = "English",

isbn = "9781628416411",

volume = "9518",

series = "Proceedings of SPIE",

publisher = "SPIE-International Society for Optical Engineering",

editor = "{van den Driesche}, {Sander }",

booktitle = "Bio-MEMS and Medical Microdevices II",

note = "SPIE Microtechnologies 2015: Bio-MEMS and Medical Microdevices II ; Conference date: 04-05-2015 Through 06-05-2015",

url = "http://spie.org/conferences-and-exhibitions/past-events/past-conferences-and-exhibitions/past-events/microtechnologies/microtechnologies-2015",

}

Belotti, Y, Conneely, M, Palmer, S, Huang, T, Campbell, PA , McKenna, S , Nabi, G & McGloin, D 2015, Hydrodynamic stretching for prostate cancer detection. in S van den Driesche (ed.), Bio-MEMS and Medical Microdevices II. vol. 9518, 95180H, Proceedings of SPIE, vol. 9518, SPIE-International Society for Optical Engineering, Bellingham, SPIE Microtechnologies 2015: Bio-MEMS and Medical Microdevices II, Barcelona, Spain, 4/05/15. https://doi.org/10.1117/12.2179201

Hydrodynamic stretching for prostate cancer detection. / Belotti, Yuri; Conneely, Michael; Palmer, Scott et al.
Bio-MEMS and Medical Microdevices II. ed. / Sander van den Driesche. Vol. 9518 Bellingham: SPIE-International Society for Optical Engineering, 2015. 95180H (Proceedings of SPIE; Vol. 9518).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Hydrodynamic stretching for prostate cancer detection

AU - Belotti, Yuri

AU - Conneely, Michael

AU - Palmer, Scott

AU - Huang, Tianjun

AU - Campbell, Paul Andrew

AU - McKenna, Stephen

AU - Nabi, Ghulam

AU - McGloin, David

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Advances in diagnostic technologies enabled scientists to link a large number of diseases with structural changes of the intracellular organisation. This intrinsic biophysical characteristic opened up the possibility to perform clinical assessments based on the measurement of single-cell mechanical properties. In this work, we combine microfluidics, high speed imaging and computational automatic tracking to measure the single-cell deformability of large samples of prostate cancer cells at a rate of ∼ 104cells/s. Such a high throughput accounts for the inherent heterogeneity of biological samples and enabled us to extract statistically meaningful signatures from each cell population. In addition, using our technique we investigate the effect of Latrunculin A to the cellular stiffness.

AB - Advances in diagnostic technologies enabled scientists to link a large number of diseases with structural changes of the intracellular organisation. This intrinsic biophysical characteristic opened up the possibility to perform clinical assessments based on the measurement of single-cell mechanical properties. In this work, we combine microfluidics, high speed imaging and computational automatic tracking to measure the single-cell deformability of large samples of prostate cancer cells at a rate of ∼ 104cells/s. Such a high throughput accounts for the inherent heterogeneity of biological samples and enabled us to extract statistically meaningful signatures from each cell population. In addition, using our technique we investigate the effect of Latrunculin A to the cellular stiffness.

KW - Automated Tracking

KW - Cell Mechanics

KW - High Speed Imaging

KW - High Throughput Diagnosis

KW - Microfluidics

KW - Prostate Cancer

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DO - 10.1117/12.2179201

M3 - Conference contribution

AN - SCOPUS:84939218549

SN - 9781628416411

VL - 9518

T3 - Proceedings of SPIE

BT - Bio-MEMS and Medical Microdevices II

A2 - van den Driesche, Sander

PB - SPIE-International Society for Optical Engineering

CY - Bellingham

T2 - SPIE Microtechnologies 2015: Bio-MEMS and Medical Microdevices II

Y2 - 4 May 2015 through 6 May 2015

ER -

Hydrodynamic stretching for prostate cancer detection

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Development of non-invasive techniques for bladder cancer diagnosis and therapy

Sequential Recognition of Manipulation Actions Using Superpixel Group Mining

Cite this

Hydrodynamic stretching for prostate cancer detection

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Student theses

Development of non-invasive techniques for bladder cancer diagnosis and therapy

Sequential Recognition of Manipulation Actions Using Superpixel Group Mining

Cite this