Hypoxic activation of an amiloride-sensitive cation conductance in alveolar epithelial cells

Sofija Jovanovic, Stephen C. Land, Richard E. Olver, Stuart M. Wilson

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Imposing hypoxia (P(O(2)) = 23 mmHg) upon A549 cells elicited increased G(amil) although previous work had predicted a fall in this parameter. G(amil) appeared to be dependent upon glucocorticoid-driven gene expression, a process inhibited by ERK, an enzyme activated by oxidative stress. However, hypoxia transiently activated this enzyme and the response was blocked by glucocorticoids, showing that the rise in G(amil) occurs only if ERK activation is suppressed. Fluorimetric assays showed that lowering P(O(2)) elicited H(2)O(2) formation indicating that this maneuver actually imposes oxidative stress, thus explaining how hypoxia can elicit responses normally associated with a rise in P(O(2)).
    Original languageEnglish
    Pages (from-to)622-7
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume286
    Issue number3
    DOIs
    Publication statusPublished - 2001

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