APETALA2 functions as a temporal factor together with BLADE-ON-PETIOLE2 and MADS29 to control flower and grain development in barley

Jennifer Shoesmith, Charles Ugochukwu Solomon, Xiujuan Yang, Laura G. Wilkinson, Scott Sheldrick, Ewan van Eijden, Sanne Couwenberg, Laura Pugh, Mhmoud Eskan, Jennifer Stephens, Abdellah Barakate, Sinéad Drea, Kelly Houston, Matthew R. Tucker (Lead / Corresponding author), Sarah M. McKim (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

Abstract

Cereal grain develops from fertilised florets. Alterations in floret and grain development greatly influence grain yield and quality. Despite this, little is known about the underlying genetic control of these processes, especially in key temperate cereals such as barley and wheat. Using a combination of near-isogenic mutant comparisons, gene editing and genetic analyses, we reveal that HvAPETALA2 (HvAP2) controls floret organ identity, floret boundaries, and maternal tissue differentiation and elimination during grain development. These new roles of HvAP2 correlate with changes in grain size and HvAP2-dependent expression of specific HvMADS-box genes, including the B-sister gene, HvMADS29. Consistent with this, gene editing demonstrates that HvMADS29 shares roles with HvAP2 in maternal tissue differentiation. We also discovered that a gain-of-function HvAP2 allele masks changes in floret organ identity and grain size due to loss of barley LAXATUM.A/BLADE-ON-PETIOLE2 (HvBOP2) gene function. Taken together, we reveal novel, pleiotropic roles and regulatory interactions for an APETALA2-like gene controlling floret and grain development in a temperate cereal.
Original languageEnglish
JournalDevelopment
Early online date1 Feb 2021
DOIs
Publication statusE-pub ahead of print - 1 Feb 2021

Keywords

  • Barley
  • AP2
  • Floret
  • Grain
  • CRISPR/Cas9
  • MADS-box
  • BOP1/2
  • B-sister
  • nucellus
  • integument

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