Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2

Natalie S. Delany, Michael Hurle, Paul Facer, Tanja Alnadaf, Christopher Plumpton, Ian Kinghorn, Chee Gee See, Michael Costigan, Praveen Anand, Clifford J. Woolf, Daniel Crowther, Philippe Sanseau, Simon N. Tate

Research output: Contribution to journalArticlepeer-review

208 Citations (Scopus)


Remarkable progress has been made recently in identifying a new gene family related to the capsaicin (vanilloid) receptor, VR1. Using a combination of in silico analysis of expressed sequence tag (EST) databases and conventional molecular cloning, we have isolated a novel vanilloid-like receptor, which we call VRL-2, from human kidney. The translated gene shares 46% and 43% identity with VR1 and VRL-1, respectively, and maps to chromosome 12q23-24.1, a locus associated with bipolar affective disorder. VRL-2 mRNA was most strongly expressed in the trachea, kidney, and salivary gland. An affinity-purified antibody against a peptide incorporating the COOH terminal of the receptor localized VRL-2 immunolabel in the distal tubules of the kidney, the epithelial linings of both trachea and lung airways, serous cells of submucosal glands, and mononuclear cells. Unlike VR1 and VRL-1, VRL-2 was not detected in cell bodies of dorsal root ganglia (DRG) or sensory nerve fibers. However, VRL-2 was found on sympathetic and parasympathetic nerve fibers, such as those innervating the arrector pili smooth muscle in skin, sweat glands, intestine, and blood vessels. At least four vanilloid receptor-like genes exist, the newest member, VRL-2 is found in airway and kidney epithelia and in the autonomic nervous system.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalPhysiological Genomics
Issue number4
Early online date19 Jan 2001
Publication statusPublished - 1 Feb 2001


  • Autonomic nervous system
  • Capsaicin
  • Dorsal root ganglia
  • Expressed sequence tag

ASJC Scopus subject areas

  • Physiology
  • Genetics


Dive into the research topics of 'Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2'. Together they form a unique fingerprint.

Cite this