Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2

Natalie S. Delany, Michael Hurle, Paul Facer, Tanja Alnadaf, Christopher Plumpton, Ian Kinghorn, Chee Gee See, Michael Costigan, Praveen Anand, Clifford J. Woolf, Daniel Crowther, Philippe Sanseau, Simon N. Tate

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Abstract

Remarkable progress has been made recently in identifying a new gene family related to the capsaicin (vanilloid) receptor, VR1. Using a combination of in silico analysis of expressed sequence tag (EST) databases and conventional molecular cloning, we have isolated a novel vanilloid-like receptor, which we call VRL-2, from human kidney. The translated gene shares 46% and 43% identity with VR1 and VRL-1, respectively, and maps to chromosome 12q23-24.1, a locus associated with bipolar affective disorder. VRL-2 mRNA was most strongly expressed in the trachea, kidney, and salivary gland. An affinity-purified antibody against a peptide incorporating the COOH terminal of the receptor localized VRL-2 immunolabel in the distal tubules of the kidney, the epithelial linings of both trachea and lung airways, serous cells of submucosal glands, and mononuclear cells. Unlike VR1 and VRL-1, VRL-2 was not detected in cell bodies of dorsal root ganglia (DRG) or sensory nerve fibers. However, VRL-2 was found on sympathetic and parasympathetic nerve fibers, such as those innervating the arrector pili smooth muscle in skin, sweat glands, intestine, and blood vessels. At least four vanilloid receptor-like genes exist, the newest member, VRL-2 is found in airway and kidney epithelia and in the autonomic nervous system.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalPhysiological Genomics
Volume2001
Issue number4
Early online date19 Jan 2001
DOIs
Publication statusPublished - 1 Feb 2001

Keywords

  • Autonomic nervous system
  • Capsaicin
  • Dorsal root ganglia
  • Expressed sequence tag

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    Delany, N. S., Hurle, M., Facer, P., Alnadaf, T., Plumpton, C., Kinghorn, I., See, C. G., Costigan, M., Anand, P., Woolf, C. J., Crowther, D., Sanseau, P., & Tate, S. N. (2001). Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2. Physiological Genomics, 2001(4), 165-174. https://doi.org/10.1152/physiolgenomics.2001.4.3.165