TY - JOUR
T1 - Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2
AU - Delany, Natalie S.
AU - Hurle, Michael
AU - Facer, Paul
AU - Alnadaf, Tanja
AU - Plumpton, Christopher
AU - Kinghorn, Ian
AU - See, Chee Gee
AU - Costigan, Michael
AU - Anand, Praveen
AU - Woolf, Clifford J.
AU - Crowther, Daniel
AU - Sanseau, Philippe
AU - Tate, Simon N.
PY - 2001/2/1
Y1 - 2001/2/1
N2 - Remarkable progress has been made recently in identifying a new gene family related to the capsaicin (vanilloid) receptor, VR1. Using a combination of in silico analysis of expressed sequence tag (EST) databases and conventional molecular cloning, we have isolated a novel vanilloid-like receptor, which we call VRL-2, from human kidney. The translated gene shares 46% and 43% identity with VR1 and VRL-1, respectively, and maps to chromosome 12q23-24.1, a locus associated with bipolar affective disorder. VRL-2 mRNA was most strongly expressed in the trachea, kidney, and salivary gland. An affinity-purified antibody against a peptide incorporating the COOH terminal of the receptor localized VRL-2 immunolabel in the distal tubules of the kidney, the epithelial linings of both trachea and lung airways, serous cells of submucosal glands, and mononuclear cells. Unlike VR1 and VRL-1, VRL-2 was not detected in cell bodies of dorsal root ganglia (DRG) or sensory nerve fibers. However, VRL-2 was found on sympathetic and parasympathetic nerve fibers, such as those innervating the arrector pili smooth muscle in skin, sweat glands, intestine, and blood vessels. At least four vanilloid receptor-like genes exist, the newest member, VRL-2 is found in airway and kidney epithelia and in the autonomic nervous system.
AB - Remarkable progress has been made recently in identifying a new gene family related to the capsaicin (vanilloid) receptor, VR1. Using a combination of in silico analysis of expressed sequence tag (EST) databases and conventional molecular cloning, we have isolated a novel vanilloid-like receptor, which we call VRL-2, from human kidney. The translated gene shares 46% and 43% identity with VR1 and VRL-1, respectively, and maps to chromosome 12q23-24.1, a locus associated with bipolar affective disorder. VRL-2 mRNA was most strongly expressed in the trachea, kidney, and salivary gland. An affinity-purified antibody against a peptide incorporating the COOH terminal of the receptor localized VRL-2 immunolabel in the distal tubules of the kidney, the epithelial linings of both trachea and lung airways, serous cells of submucosal glands, and mononuclear cells. Unlike VR1 and VRL-1, VRL-2 was not detected in cell bodies of dorsal root ganglia (DRG) or sensory nerve fibers. However, VRL-2 was found on sympathetic and parasympathetic nerve fibers, such as those innervating the arrector pili smooth muscle in skin, sweat glands, intestine, and blood vessels. At least four vanilloid receptor-like genes exist, the newest member, VRL-2 is found in airway and kidney epithelia and in the autonomic nervous system.
KW - Autonomic nervous system
KW - Capsaicin
KW - Dorsal root ganglia
KW - Expressed sequence tag
UR - http://www.scopus.com/inward/record.url?scp=0348142491&partnerID=8YFLogxK
U2 - 10.1152/physiolgenomics.2001.4.3.165
DO - 10.1152/physiolgenomics.2001.4.3.165
M3 - Article
AN - SCOPUS:0348142491
SN - 1531-2267
VL - 2001
SP - 165
EP - 174
JO - Physiological Genomics
JF - Physiological Genomics
IS - 4
ER -