Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum

Jie Li; Aman Kaur; Brian Harrower; Miles Armstrong; Daolong Dou; Xiaodan Wang; Ingo Hein

doi:10.1016/j.cj.2023.08.005

Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum

Jie Li, Aman Kaur, Brian Harrower, Miles Armstrong, Daolong Dou, Xiaodan Wang (Lead / Corresponding author), Ingo Hein (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen, Phytophthora infestans. We have identified and mapped a novel broad-spectrum disease resistance gene effective against P. infestans from the wild, diploid potato species Solanum bulbocastanum. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to as Rpi-blb4, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.

Original language	English
Number of pages	8
Journal	The Crop Journal
Early online date	24 Sept 2023
DOIs	https://doi.org/10.1016/j.cj.2023.08.005
Publication status	E-pub ahead of print - 24 Sept 2023

Keywords

Renseq
Disease Resistance
Late Blight
Solanum Bulbocastanum
Disease resistance
Late blight
Solanum bulbocastanum

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1016/j.cj.2023.08.005Licence: CC BY-NC-ND

Target Enrichment-Based Potato Disease Resistance Gene Studies and Late-Blight Pathogen Diversity Research (Joint with China Agricultural University) (Newton Advanced Fellowship 2020)
Hein, I.
31/03/20 → 30/03/22
Project: Research
Studying Co Evolution in Agriculture to Inform NLR Deployment (Industrial Partnership Award - IPA)
Hein, I.
Biotechnology and Biological Sciences Research Council
1/12/19 → 31/08/23
Project: Research

Cite this

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title = "Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum",

abstract = "More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen, Phytophthora infestans. We have identified and mapped a novel broad-spectrum disease resistance gene effective against P. infestans from the wild, diploid potato species Solanum bulbocastanum. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to as Rpi-blb4, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.",

keywords = "Renseq, Disease Resistance, Late Blight, Solanum Bulbocastanum, Disease resistance, Late blight, Solanum bulbocastanum",

author = "Jie Li and Aman Kaur and Brian Harrower and Miles Armstrong and Daolong Dou and Xiaodan Wang and Ingo Hein",

note = "Funding Information: This work was supported by the Rural & Environment Science & Analytical Services (RESAS) Division of the Scottish Government (JHI-B1-1), the Biotechnology and Biological Sciences Research Council (BBSRC, BB/S015663/1), the Royal Society (NAF\R1\201061), and the National Natural Science Foundation of China (32061130211, 32372558). AK was supported through a Research Leaders 2025 fellowship funded by European Union{\textquoteright}s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie Grant Agreement (754380). The authors acknowledge the Research/Scientific Computing teams at The James Hutton Institute and NIAB for providing computational resources and technical support for the “UK{\textquoteright}s Crop Diversity Bioinformatics HPC” (BBSRC Grant BB/S019669/1), use of which has contributed to the results reported within this paper. Copyright: {\textcopyright} 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.",

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AU - Li, Jie

AU - Kaur, Aman

AU - Harrower, Brian

AU - Armstrong, Miles

AU - Dou, Daolong

AU - Wang, Xiaodan

AU - Hein, Ingo

N1 - Funding Information: This work was supported by the Rural & Environment Science & Analytical Services (RESAS) Division of the Scottish Government (JHI-B1-1), the Biotechnology and Biological Sciences Research Council (BBSRC, BB/S015663/1), the Royal Society (NAF\R1\201061), and the National Natural Science Foundation of China (32061130211, 32372558). AK was supported through a Research Leaders 2025 fellowship funded by European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie Grant Agreement (754380). The authors acknowledge the Research/Scientific Computing teams at The James Hutton Institute and NIAB for providing computational resources and technical support for the “UK’s Crop Diversity Bioinformatics HPC” (BBSRC Grant BB/S019669/1), use of which has contributed to the results reported within this paper. Copyright: © 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.

PY - 2023/9/24

Y1 - 2023/9/24

N2 - More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen, Phytophthora infestans. We have identified and mapped a novel broad-spectrum disease resistance gene effective against P. infestans from the wild, diploid potato species Solanum bulbocastanum. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to as Rpi-blb4, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.

AB - More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen, Phytophthora infestans. We have identified and mapped a novel broad-spectrum disease resistance gene effective against P. infestans from the wild, diploid potato species Solanum bulbocastanum. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to as Rpi-blb4, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.

KW - Renseq

KW - Disease Resistance

KW - Late Blight

KW - Solanum Bulbocastanum

KW - Disease resistance

KW - Late blight

KW - Solanum bulbocastanum

U2 - 10.1016/j.cj.2023.08.005

DO - 10.1016/j.cj.2023.08.005

M3 - Article

SN - 2095-5421

JO - The Crop Journal

JF - The Crop Journal

ER -

Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum

Abstract

Keywords

ASJC Scopus subject areas

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Projects

Target Enrichment-Based Potato Disease Resistance Gene Studies and Late-Blight Pathogen Diversity Research (Joint with China Agricultural University) (Newton Advanced Fellowship 2020)

Studying Co Evolution in Agriculture to Inform NLR Deployment (Industrial Partnership Award - IPA)

Cite this