Identification of a basic hybrid glutathione S-transferase from human liver. Glutathione S-transferase delta is composed of two distinct subunits (B1 and B2)

P K Stockman, G J Beckett, J D Hayes

    Research output: Contribution to journalArticlepeer-review

    69 Citations (Scopus)

    Abstract

    The purification of a hybrid glutathione S-transferase (B1 B2) from human liver is described. This enzyme has an isoelectric point of 8.75 and the B1 and B2 subunits are distinguishable immunologically and are ionically distinct. Hybridization experiments demonstrated that B1 B1 and B2 B2 could be resolved by CM-cellulose chromatography and have pI values of 8.9 and 8.4 respectively. Transferase B1 B2, and the two homodimers from which it is formed, are electrophoretically and immunochemically distinct from the neutral enzyme (transferase mu) and two acidic enzymes (transferases rho and lambda). Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis demonstrated that B1 and B2 both have an Mr of 26 000, whereas, in contrast, transferase mu comprises subunits of Mr 27 000 and transferases rho and lambda both comprise subunits of Mr 24 500. Antisera raised against B1 or B2 monomers did not cross-react with the neutral or acidic glutathione S-transferases. The identity of transferase B1 B2 with glutathione S-transferase delta prepared by the method of Kamisaka, Habig, Ketley, Arias & Jakoby [(1975) Eur. J. Biochem. 60, 153-161] has been demonstrated, as well as its relationship to other previously described transferases.

    Original languageEnglish
    Pages (from-to)457-65
    Number of pages9
    JournalBiochemical Journal
    Volume227
    Issue number2
    Publication statusPublished - 15 Apr 1985

    Keywords

    • Chromatography, Affinity
    • Chromatography, Ion Exchange
    • Glutathione Transferase/immunology
    • Humans
    • Isoenzymes/immunology
    • Liver/enzymology

    Fingerprint

    Dive into the research topics of 'Identification of a basic hybrid glutathione S-transferase from human liver. Glutathione S-transferase delta is composed of two distinct subunits (B1 and B2)'. Together they form a unique fingerprint.

    Cite this