Identification of a calmodulin-dependent glycogen synthase kinase in rabbit skeletal muscle, distinct from phosphorylase kinase

James R. Woodgett, Nicholas K. Tonks, Philip Cohen

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    Abstract

    A glycogen synthase kinase that is completely dependent on Ca2+ and calmodulin has been identified in mammalian skeletal muscle, and purified ∼3000-fold by chromatography on phosphocellulose and calmodulin-Sepharose. The presence of 50 mM NaCl in the homogenisation buffer was critical for extraction of the enzyme. The calmodulin-dependent glycogen synthase kinase (app. Mr 850 000) is distinct from myosin light-chain kinase and phosphorylase kinase, but phosphorylates the same serine residue on glycogen synthase as phosphorylase kinase. The physiological role of the enzyme is discussed.

    Original languageEnglish
    Pages (from-to)5-11
    Number of pages7
    JournalFEBS Letters
    Volume148
    Issue number1
    DOIs
    Publication statusPublished - 1 Nov 1982

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    Keywords

    • Calmodulin
    • Cyclic AMP
    • Glycogen synthase
    • Protein phosphorylation

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