Identification of a calmodulin-dependent glycogen synthase kinase in rabbit skeletal muscle, distinct from phosphorylase kinase

James R. Woodgett; Nicholas K. Tonks; Philip Cohen

doi:10.1016/0014-5793(82)81231-3

Identification of a calmodulin-dependent glycogen synthase kinase in rabbit skeletal muscle, distinct from phosphorylase kinase

James R. Woodgett, Nicholas K. Tonks, Philip Cohen

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Abstract

A glycogen synthase kinase that is completely dependent on Ca²⁺ and calmodulin has been identified in mammalian skeletal muscle, and purified ∼3000-fold by chromatography on phosphocellulose and calmodulin-Sepharose. The presence of 50 mM NaCl in the homogenisation buffer was critical for extraction of the enzyme. The calmodulin-dependent glycogen synthase kinase (app. M_r 850 000) is distinct from myosin light-chain kinase and phosphorylase kinase, but phosphorylates the same serine residue on glycogen synthase as phosphorylase kinase. The physiological role of the enzyme is discussed.

Original language	English
Pages (from-to)	5-11
Number of pages	7
Journal	FEBS Letters
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(82)81231-3
Publication status	Published - 1 Nov 1982

Keywords

Calmodulin
Cyclic AMP
Glycogen synthase
Protein phosphorylation

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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AU - Woodgett, James R.

AU - Tonks, Nicholas K.

AU - Cohen, Philip

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Y1 - 1982/11/1

N2 - A glycogen synthase kinase that is completely dependent on Ca2+ and calmodulin has been identified in mammalian skeletal muscle, and purified ∼3000-fold by chromatography on phosphocellulose and calmodulin-Sepharose. The presence of 50 mM NaCl in the homogenisation buffer was critical for extraction of the enzyme. The calmodulin-dependent glycogen synthase kinase (app. Mr 850 000) is distinct from myosin light-chain kinase and phosphorylase kinase, but phosphorylates the same serine residue on glycogen synthase as phosphorylase kinase. The physiological role of the enzyme is discussed.

AB - A glycogen synthase kinase that is completely dependent on Ca2+ and calmodulin has been identified in mammalian skeletal muscle, and purified ∼3000-fold by chromatography on phosphocellulose and calmodulin-Sepharose. The presence of 50 mM NaCl in the homogenisation buffer was critical for extraction of the enzyme. The calmodulin-dependent glycogen synthase kinase (app. Mr 850 000) is distinct from myosin light-chain kinase and phosphorylase kinase, but phosphorylates the same serine residue on glycogen synthase as phosphorylase kinase. The physiological role of the enzyme is discussed.

KW - Calmodulin

KW - Cyclic AMP

KW - Glycogen synthase

KW - Protein phosphorylation

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ER -

Identification of a calmodulin-dependent glycogen synthase kinase in rabbit skeletal muscle, distinct from phosphorylase kinase

Abstract

Keywords

ASJC Scopus subject areas

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