Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf

Sarah Traverse (Lead / Corresponding author), Philip Cohen

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after stimulation of PC12 cells with nerve growth factor or epidermal growth factor, but this is not accompanied by activation of the Mono Q-purified enzyme whether assayed in the absence or presence of GTP-Ras.

    Original languageEnglish
    Pages (from-to)13-18
    Number of pages6
    JournalFEBS Letters
    Volume350
    Issue number1
    DOIs
    Publication statusPublished - 15 Aug 1994

    Keywords

    • Epidermal growth factor
    • MAP kinase
    • Nerve growth factor
    • PC12 cell
    • Raf

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