Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf

Sarah Traverse; Philip Cohen

doi:10.1016/0014-5793(94)00723-3

Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf

Sarah Traverse (Lead / Corresponding author), Philip Cohen

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after stimulation of PC12 cells with nerve growth factor or epidermal growth factor, but this is not accompanied by activation of the Mono Q-purified enzyme whether assayed in the absence or presence of GTP-Ras.

Original language	English
Pages (from-to)	13-18
Number of pages	6
Journal	FEBS Letters
Volume	350
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)00723-3
Publication status	Published - 15 Aug 1994

Keywords

Epidermal growth factor
MAP kinase
Nerve growth factor
PC12 cell
Raf

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Molecular Biology
Genetics
Cell Biology

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KW - Epidermal growth factor

KW - MAP kinase

KW - Nerve growth factor

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Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf

Abstract

Keywords

ASJC Scopus subject areas

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