Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf

TY - JOUR

T1 - Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf

AU - Traverse, Sarah

AU - Cohen, Philip

PY - 1994/8/15

Y1 - 1994/8/15

N2 - A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after stimulation of PC12 cells with nerve growth factor or epidermal growth factor, but this is not accompanied by activation of the Mono Q-purified enzyme whether assayed in the absence or presence of GTP-Ras.

AB - A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after stimulation of PC12 cells with nerve growth factor or epidermal growth factor, but this is not accompanied by activation of the Mono Q-purified enzyme whether assayed in the absence or presence of GTP-Ras.

KW - Epidermal growth factor

KW - MAP kinase

KW - Nerve growth factor

KW - PC12 cell

KW - Raf

UR - https://www.scopus.com/pages/publications/0027930493

U2 - 10.1016/0014-5793(94)00723-3

DO - 10.1016/0014-5793(94)00723-3

M3 - Article

C2 - 8062910

AN - SCOPUS:0027930493

SN - 0014-5793

VL - 350

SP - 13

EP - 18

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -