Identification of a regulatory phosphorylation site in the hinge 1 region of nitrate reductase from spinach (Spinacea oleracea) leaves

TY - JOUR

T1 - Identification of a regulatory phosphorylation site in the hinge 1 region of nitrate reductase from spinach (Spinacea oleracea) leaves

AU - Douglas, Pauline

AU - Morrice, Nick

AU - MacKintosh, Carol

PY - 1995

Y1 - 1995

N2 - Purified nitrate reductase (NR) from spinach leaves was phosphorylated in vitro by NR-inactivating kinase on Ser-543 which is located in the hinge 1 region between the molybdenum-cofactor and haem-binding domains, Phosphorylation of Ser-543 allowed NR to be inhibited by the inhibitor, NIP, Degraded NR preparations in which a proportion of the subunits had lost 45 amino acids from the N-terminus during purification could be phosphorylated by NR kinase on Ser-543, but could not subsequently be fully inhibited by NIP, suggesting a role for the N-terminal tail of NR in NIP binding.

AB - Purified nitrate reductase (NR) from spinach leaves was phosphorylated in vitro by NR-inactivating kinase on Ser-543 which is located in the hinge 1 region between the molybdenum-cofactor and haem-binding domains, Phosphorylation of Ser-543 allowed NR to be inhibited by the inhibitor, NIP, Degraded NR preparations in which a proportion of the subunits had lost 45 amino acids from the N-terminus during purification could be phosphorylated by NR kinase on Ser-543, but could not subsequently be fully inhibited by NIP, suggesting a role for the N-terminal tail of NR in NIP binding.

M3 - Article

SN - 0014-5793

VL - 377

SP - 113

EP - 117

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -