Identification of a target for CudA, the transcription factor which directs formation of the Dictyostelium tip organiser

Hong-Yu Wang, Jeffrey G. Williams

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    7 Citations (Scopus)


    The tip of the Dictyostelium slug functions much like an embryonic organiser; when grafted onto the flank of a recipient slug, it recruits amass of prespore cells and leads them away as part of a secondary slug. CudA is a nuclear protein which is expressed in prespore cells where it acts as a specific transcription factor. CudA is also expressed in an anteriorly located group of cells, the tip-organiser, that is believed to constitute the functional tip. We identify an expansin-like gene, exp17, that is expressed within the tip-organiser region and which is not expressed in a cudA null strain. The exp17 promoter contains a region that binds to CudA in vitro and this region is necessary for expression in the tip-organiser. These results provide an end-point for a previously defined signal transduction pathway in which regionalised expression of the ACA adenylyl cyclase within the tip-organiser leads to localised cAMP-induced activation of STATa and consequent binding of STATa to the cudA promoter. STATa then induces expression of cudA and cudA directs the transcription of target genes such as exp17.

    Original languageEnglish
    Pages (from-to)161-165
    Number of pages5
    JournalInternational Journal of Developmental Biology
    Issue number1
    Publication statusPublished - 2010


    • Dictyostelium
    • CudA
    • Tip
    • Expansin
    • Gene expression
    • In situ hybridization
    • Gene expression
    • Discoideum
    • Patterns
    • Switch
    • Slugs

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