Identification of catabolite repression as a physiological regulator of biofilm formation by Bacillus subtilis by use of DNA microarrays

Nicola R. Stanley, Robert A. Britton, Alan D. Grossman, Beth A. Lazazzera

    Research output: Contribution to journalArticle

    176 Citations (Scopus)

    Abstract

    Biofilms are structured communities of cells that are encased in a self-produced polymeric matrix and are adherent to a surface. Many biofilms have a significant impact in medical and industrial settings. The model gram-positive bacterium Bacillus subtilis has recently been shown to form biofilms. To gain insight into the genes involved in biofilm formation by this bacterium, we used DNA microarrays representing >99% of the annotated B. subtilis open reading frames to follow the temporal changes in gene expression that occurred as cells transitioned from a planktonic to a biofilm state. We identified 519 genes that were differentially expressed at one or more time points as cells transitioned to a biofilm. Approximately 6% of the genes of B. subtilis were differentially expressed at a time when 98% of the cells in the population were in a biofilm. These genes were involved in motility, phage-related functions, and metabolism. By comparing the genes differentially expressed during biofilm formation with those identified in other genomewide transcriptional-profiling studies, we were able to identify several transcription factors whose activities appeared to be altered during the transition from a planktonic state to a biofilm. Two of these transcription factors were Spo0A and sigma-H, which had previously been shown to affect biofilm formation by B. subtilis. A third signal that appeared to be affecting gene expression during biofilm formation was glucose depletion. Through quantitative biofilm assays and confocal scanning laser microscopy, we observed that glucose inhibited biofilm formation through the catabolite control protein CcpA.
    Original languageEnglish
    Pages (from-to)1951-1957
    Number of pages7
    JournalJournal of Bacteriology
    Volume185
    Issue number6
    DOIs
    Publication statusPublished - Mar 2003

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    Catabolite Repression
    Biofilms
    Bacillus subtilis
    Oligonucleotide Array Sequence Analysis
    Genes
    Transcription Factors
    Gene Expression
    Glucose
    Gram-Positive Bacteria
    Confocal Microscopy
    Bacteriophages
    Open Reading Frames

    Cite this

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    title = "Identification of catabolite repression as a physiological regulator of biofilm formation by Bacillus subtilis by use of DNA microarrays",
    abstract = "Biofilms are structured communities of cells that are encased in a self-produced polymeric matrix and are adherent to a surface. Many biofilms have a significant impact in medical and industrial settings. The model gram-positive bacterium Bacillus subtilis has recently been shown to form biofilms. To gain insight into the genes involved in biofilm formation by this bacterium, we used DNA microarrays representing >99{\%} of the annotated B. subtilis open reading frames to follow the temporal changes in gene expression that occurred as cells transitioned from a planktonic to a biofilm state. We identified 519 genes that were differentially expressed at one or more time points as cells transitioned to a biofilm. Approximately 6{\%} of the genes of B. subtilis were differentially expressed at a time when 98{\%} of the cells in the population were in a biofilm. These genes were involved in motility, phage-related functions, and metabolism. By comparing the genes differentially expressed during biofilm formation with those identified in other genomewide transcriptional-profiling studies, we were able to identify several transcription factors whose activities appeared to be altered during the transition from a planktonic state to a biofilm. Two of these transcription factors were Spo0A and sigma-H, which had previously been shown to affect biofilm formation by B. subtilis. A third signal that appeared to be affecting gene expression during biofilm formation was glucose depletion. Through quantitative biofilm assays and confocal scanning laser microscopy, we observed that glucose inhibited biofilm formation through the catabolite control protein CcpA.",
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    Identification of catabolite repression as a physiological regulator of biofilm formation by Bacillus subtilis by use of DNA microarrays. / Stanley, Nicola R.; Britton, Robert A.; Grossman, Alan D.; Lazazzera, Beth A.

    In: Journal of Bacteriology, Vol. 185, No. 6, 03.2003, p. 1951-1957.

    Research output: Contribution to journalArticle

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    T1 - Identification of catabolite repression as a physiological regulator of biofilm formation by Bacillus subtilis by use of DNA microarrays

    AU - Stanley, Nicola R.

    AU - Britton, Robert A.

    AU - Grossman, Alan D.

    AU - Lazazzera, Beth A.

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