Abstract
Import of proteins into the nucleus is a two-step process, involving nuclear localization sequence (NLS)-dependent docking of the substrate at the nuclear envelope followed by translocation through the nuclear pore. A recombinant human protein, hSRP1α, bound in vitro specifically and directly to substrates containing either a simple or bipartite NLS motif. hSRP1α promoted docking of import substrates to the nuclear envelope and together with recombinant human Ran reconstituted complete nuclear protein import. Thus, hSRP1α has the properties of a cytosolic receptor for both simple and bipartite NLS motifs.
Original language | English |
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Pages (from-to) | 1049-1053 |
Number of pages | 5 |
Journal | Science |
Volume | 268 |
Issue number | 5213 |
DOIs | |
Publication status | Published - 19 May 1995 |
ASJC Scopus subject areas
- General