Identification of hSRP1α as a functional receptor for nuclear localization sequences

TY - JOUR

T1 - Identification of hSRP1α as a functional receptor for nuclear localization sequences

AU - Weis, Karsten

AU - Mattaj, Iain W.

AU - Lamond, Angus I.

PY - 1995/5/19

Y1 - 1995/5/19

N2 - Import of proteins into the nucleus is a two-step process, involving nuclear localization sequence (NLS)-dependent docking of the substrate at the nuclear envelope followed by translocation through the nuclear pore. A recombinant human protein, hSRP1α, bound in vitro specifically and directly to substrates containing either a simple or bipartite NLS motif. hSRP1α promoted docking of import substrates to the nuclear envelope and together with recombinant human Ran reconstituted complete nuclear protein import. Thus, hSRP1α has the properties of a cytosolic receptor for both simple and bipartite NLS motifs.

AB - Import of proteins into the nucleus is a two-step process, involving nuclear localization sequence (NLS)-dependent docking of the substrate at the nuclear envelope followed by translocation through the nuclear pore. A recombinant human protein, hSRP1α, bound in vitro specifically and directly to substrates containing either a simple or bipartite NLS motif. hSRP1α promoted docking of import substrates to the nuclear envelope and together with recombinant human Ran reconstituted complete nuclear protein import. Thus, hSRP1α has the properties of a cytosolic receptor for both simple and bipartite NLS motifs.

UR - https://www.scopus.com/pages/publications/0028988731

U2 - 10.1126/science.7754385

DO - 10.1126/science.7754385

M3 - Article

C2 - 7754385

AN - SCOPUS:0028988731

SN - 0036-8075

VL - 268

SP - 1049

EP - 1053

JO - Science

JF - Science

IS - 5213

ER -