Identification of human miRNA precursors that resemble box C/D snoRNAs

Motoharu Ono, Michelle S. Scott, Kayo Yamada, Fabio Avolio, Geoffrey J. Barton, Angus I. Lamond

    Research output: Contribution to journalArticle

    88 Citations (Scopus)

    Abstract

    There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigate a similar evolutionary relationship between a subset of miRNA precursors and box C/D snoRNAs. Computational analyses identified 84 intronic miRNAs that are encoded within either box C/D snoRNAs, or in precursors showing similarity to box C/D snoRNAs. Predictions of the folded structures of these box C/D snoRNA-like miRNA precursors resemble the structures of known box C/D snoRNAs, with the boxes C and D often in close proximity in the folded molecule. All five box C/D snoRNA-like miRNA precursors tested (miR-27b, miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional box C/D snoRNP complexes. The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs.

    Original languageEnglish
    Pages (from-to)3879-3891
    Number of pages13
    JournalNucleic Acids Research
    Volume39
    Issue number9
    DOIs
    Publication statusPublished - May 2011

    Keywords

    • RNA SECONDARY STRUCTURE
    • SMALL NUCLEOLAR RNAS
    • HOST GENES
    • MICRORNAS
    • NUCLEAR
    • CANDIDATES
    • DYNAMICS
    • REVEALS
    • MOUSE
    • CELLS

    Cite this

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    title = "Identification of human miRNA precursors that resemble box C/D snoRNAs",
    abstract = "There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigate a similar evolutionary relationship between a subset of miRNA precursors and box C/D snoRNAs. Computational analyses identified 84 intronic miRNAs that are encoded within either box C/D snoRNAs, or in precursors showing similarity to box C/D snoRNAs. Predictions of the folded structures of these box C/D snoRNA-like miRNA precursors resemble the structures of known box C/D snoRNAs, with the boxes C and D often in close proximity in the folded molecule. All five box C/D snoRNA-like miRNA precursors tested (miR-27b, miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional box C/D snoRNP complexes. The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs.",
    keywords = "RNA SECONDARY STRUCTURE, SMALL NUCLEOLAR RNAS, HOST GENES, MICRORNAS, NUCLEAR, CANDIDATES, DYNAMICS, REVEALS, MOUSE, CELLS",
    author = "Motoharu Ono and Scott, {Michelle S.} and Kayo Yamada and Fabio Avolio and Barton, {Geoffrey J.} and Lamond, {Angus I.}",
    year = "2011",
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    Identification of human miRNA precursors that resemble box C/D snoRNAs. / Ono, Motoharu; Scott, Michelle S.; Yamada, Kayo; Avolio, Fabio; Barton, Geoffrey J.; Lamond, Angus I.

    In: Nucleic Acids Research, Vol. 39, No. 9, 05.2011, p. 3879-3891.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Identification of human miRNA precursors that resemble box C/D snoRNAs

    AU - Ono, Motoharu

    AU - Scott, Michelle S.

    AU - Yamada, Kayo

    AU - Avolio, Fabio

    AU - Barton, Geoffrey J.

    AU - Lamond, Angus I.

    PY - 2011/5

    Y1 - 2011/5

    N2 - There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigate a similar evolutionary relationship between a subset of miRNA precursors and box C/D snoRNAs. Computational analyses identified 84 intronic miRNAs that are encoded within either box C/D snoRNAs, or in precursors showing similarity to box C/D snoRNAs. Predictions of the folded structures of these box C/D snoRNA-like miRNA precursors resemble the structures of known box C/D snoRNAs, with the boxes C and D often in close proximity in the folded molecule. All five box C/D snoRNA-like miRNA precursors tested (miR-27b, miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional box C/D snoRNP complexes. The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs.

    AB - There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigate a similar evolutionary relationship between a subset of miRNA precursors and box C/D snoRNAs. Computational analyses identified 84 intronic miRNAs that are encoded within either box C/D snoRNAs, or in precursors showing similarity to box C/D snoRNAs. Predictions of the folded structures of these box C/D snoRNA-like miRNA precursors resemble the structures of known box C/D snoRNAs, with the boxes C and D often in close proximity in the folded molecule. All five box C/D snoRNA-like miRNA precursors tested (miR-27b, miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional box C/D snoRNP complexes. The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs.

    KW - RNA SECONDARY STRUCTURE

    KW - SMALL NUCLEOLAR RNAS

    KW - HOST GENES

    KW - MICRORNAS

    KW - NUCLEAR

    KW - CANDIDATES

    KW - DYNAMICS

    KW - REVEALS

    KW - MOUSE

    KW - CELLS

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    JF - Nucleic Acids Research

    SN - 0305-1048

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    ER -