Projects per year
There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigate a similar evolutionary relationship between a subset of miRNA precursors and box C/D snoRNAs. Computational analyses identified 84 intronic miRNAs that are encoded within either box C/D snoRNAs, or in precursors showing similarity to box C/D snoRNAs. Predictions of the folded structures of these box C/D snoRNA-like miRNA precursors resemble the structures of known box C/D snoRNAs, with the boxes C and D often in close proximity in the folded molecule. All five box C/D snoRNA-like miRNA precursors tested (miR-27b, miR-16-1, mir-28, miR-31 and let-7g) bind to fibrillarin, a specific protein component of functional box C/D snoRNP complexes. The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs.
|Number of pages||13|
|Journal||Nucleic Acids Research|
|Publication status||Published - May 2011|
- RNA SECONDARY STRUCTURE
- SMALL NUCLEOLAR RNAS
- HOST GENES
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- 3 Finished
Strategic Award: Wellcome Trust Technology Platform
Blow, J., Lamond, A. & Owen-Hughes, T.
1/01/13 → 30/09/18
Aref#d: 18800. Quantitative Mass Spectrometry Analysis of Gene Expression and Protein Dynamics (Equipment application)
Blow, J., Hay, R., Hutvagner, G., Lamond, A. & Swedlow, J.
12/06/08 → 11/06/13
Aref#d: 19770. Division of Gene Regulation and Expression Strategic Award
Blow, J., Hutvagner, G., Lamond, A., Owen-Hughes, T. & Swedlow, J.
1/01/08 → 31/12/12