Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins

David Stokoe, Katrin Engel, David G. Campbell, Philip Cohen, Matthias Gaestel

    Research output: Contribution to journalArticlepeer-review

    457 Citations (Scopus)

    Abstract

    MAP kinase-activated protein kinase-2 (MAPKAP kinase-2) phosphorylates the serine residues in murine heat shock protein 25 (hsp25) and human heat shock protein 27 (hsp27) which are phosphorylated in vivo in response to growth factors and heat shock, namely Ser15 and Ser40 (hsp25) and Ser15, Ser78 and Ser82 (hsp27). Se86 of hsp25 and the equivalent residue in hsp27 (Ser82) are phosphorylated preferentially in vitro. The small heat shock protein is present in rabbit skeletal muscle and hsp25 kinase activity in skeletal muscle extracts co-purifies with MAPKAP kinase-2 activity throughout the purification of the latter enzyme. These results suggest that MAPKAP kinase-2 is the enzyme responsible for the phosphorylation of these small heat shock proteins in mammalian cells.

    Original languageEnglish
    Pages (from-to)307-313
    Number of pages7
    JournalFEBS Letters
    Volume313
    Issue number3
    DOIs
    Publication statusPublished - 30 Nov 1992

    Keywords

    • Growth factor
    • Heat shock
    • MAP kinase
    • Protein kinase
    • Protein phosphorylation

    Fingerprint Dive into the research topics of 'Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins'. Together they form a unique fingerprint.

    Cite this