Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide.

Susanne E. Zamze; Michael A. J. Ferguson; E. Richard Moxon; Raymond A. Dwek; Thomas W. Rademacher

Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide.

Susanne E. Zamze, Michael A. J. Ferguson, E. Richard Moxon, Raymond A. Dwek, Thomas W. Rademacher

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Abstract

A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb⁺169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.

Original language	English
Pages (from-to)	583-587
Number of pages	5
Journal	Biochemical Journal
Volume	245
Issue number	2
Publication status	Published - 15 Jul 1987

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title = "Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide.",

abstract = "A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.",

author = "Zamze, {Susanne E.} and Ferguson, {Michael A. J.} and Moxon, {E. Richard} and Dwek, {Raymond A.} and Rademacher, {Thomas W.}",

year = "1987",

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language = "English",

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pages = "583--587",

journal = "Biochemical Journal",

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T1 - Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide.

AU - Zamze, Susanne E.

AU - Ferguson, Michael A. J.

AU - Moxon, E. Richard

AU - Dwek, Raymond A.

AU - Rademacher, Thomas W.

PY - 1987/7/15

Y1 - 1987/7/15

N2 - A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.

AB - A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.

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M3 - Article

AN - SCOPUS:0023655513

SN - 0264-6021

VL - 245

SP - 583

EP - 587

JO - Biochemical Journal

JF - Biochemical Journal

IS - 2

ER -

Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide.

Abstract

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