Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide.

Susanne E. Zamze, Michael A. J. Ferguson, E. Richard Moxon, Raymond A. Dwek, Thomas W. Rademacher

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.

    Original languageEnglish
    Pages (from-to)583-587
    Number of pages5
    JournalBiochemical Journal
    Volume245
    Issue number2
    Publication statusPublished - 15 Jul 1987

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    Haemophilus influenzae
    Lipopolysaccharides
    Acids
    Gas chromatography
    Gas Chromatography-Mass Spectrometry
    Mass spectrometry
    Alkaline Phosphatase
    Hydrolysis

    Cite this

    Zamze, Susanne E. ; Ferguson, Michael A. J. ; Moxon, E. Richard ; Dwek, Raymond A. ; Rademacher, Thomas W. / Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide. In: Biochemical Journal. 1987 ; Vol. 245, No. 2. pp. 583-587.
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    abstract = "A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.",
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    Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide. / Zamze, Susanne E.; Ferguson, Michael A. J.; Moxon, E. Richard; Dwek, Raymond A.; Rademacher, Thomas W.

    In: Biochemical Journal, Vol. 245, No. 2, 15.07.1987, p. 583-587.

    Research output: Contribution to journalArticle

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    AU - Zamze, Susanne E.

    AU - Ferguson, Michael A. J.

    AU - Moxon, E. Richard

    AU - Dwek, Raymond A.

    AU - Rademacher, Thomas W.

    PY - 1987/7/15

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    AB - A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.

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