Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide.

Susanne E. Zamze, Michael A. J. Ferguson, E. Richard Moxon, Raymond A. Dwek, Thomas W. Rademacher

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.

    Original languageEnglish
    Pages (from-to)583-587
    Number of pages5
    JournalBiochemical Journal
    Volume245
    Issue number2
    Publication statusPublished - 15 Jul 1987

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