Abstract
In the cytosol of A. salina cryptobiotic gastrulae at least five protein phosphatases active on phosphorylase a have been detected by ion exchange chromatography on DEAE-cellulose. Only two of these enzymes (PP-X and PP-Y) are active in mRNP dephosphorylation. Both enzymes are insensitive to inhibitor-1 and -2 and stimulation of enzymatic activity (2.5-fold with PP-X and 6.5-fold with PP-Y) can be accomplished by ethanol treatment of the native enzymes, or freeze-thawing in the presence of 1.7% ( v v) 2-mercaptoethanol. These properties allow PP-X and PP-Y to be classified as type-2A enzymes according to the nomenclature of Cohen. This paper is the first report of protein phosphatases capable of dephosphorylating mRNP proteins.
Original language | English |
---|---|
Pages (from-to) | 84-90 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 131 |
Issue number | 1 |
DOIs | |
Publication status | Published - 30 Aug 1985 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology