Identification of QTLs associated with potassium use efficiency and underlying candidate genes by whole-genome resequencing of two parental lines in Brassica napus

Wei Wang; Jinsong Zou; Philip J White; Guangda Ding; Yalin Li; Fangsen Xu; Lei Shi

doi:10.1016/j.ygeno.2021.01.020

Identification of QTLs associated with potassium use efficiency and underlying candidate genes by whole-genome resequencing of two parental lines in Brassica napus

Wei Wang, Jinsong Zou, Philip J White, Guangda Ding, Yalin Li, Fangsen Xu, Lei Shi (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Breeding crops that acquire and/or utilize potassium (K) more effectively could reduce the use of K fertilizers. Sixteen traits affecting K use efficiency (KUE) at the seedling stage were investigated in a B. napus double haploid population grown at an optimal K supply (OK) and a low K supply (LK) in a hydroponic culture system. In total, 50 and 62 QTLs associated with these traits were identified at OK and LK, respectively. A total of 25 orthologues of 23 Arabidopsis genes regulating K transport were identified in the confidence intervals of nine QTLs impacting shoot dry weight at LK, and 22 of these showed variations in coding sequences and/or exhibited significant differences in mRNA abundances in roots at LK between the two parental lines. This study provided insights to the genetic basis of KUE in B. napus, which will accelerate the breeding of K-efficient rapeseed cultivars by marker-assisted selection.

Original language	English
Pages (from-to)	755-768
Number of pages	14
Journal	Genomics
Volume	113
Issue number	2
Early online date	29 Jan 2021
DOIs	https://doi.org/10.1016/j.ygeno.2021.01.020
Publication status	Published - Mar 2021

Keywords

Brassica napus/genetics
INDEL Mutation
Ion Transport
Plant Proteins/genetics
Polymorphism, Single Nucleotide
Potassium/metabolism
Quantitative Trait Loci

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@article{c7f8bc3732364919bb3b22fe86c4b49d,

title = "Identification of QTLs associated with potassium use efficiency and underlying candidate genes by whole-genome resequencing of two parental lines in Brassica napus",

abstract = "Breeding crops that acquire and/or utilize potassium (K) more effectively could reduce the use of K fertilizers. Sixteen traits affecting K use efficiency (KUE) at the seedling stage were investigated in a B. napus double haploid population grown at an optimal K supply (OK) and a low K supply (LK) in a hydroponic culture system. In total, 50 and 62 QTLs associated with these traits were identified at OK and LK, respectively. A total of 25 orthologues of 23 Arabidopsis genes regulating K transport were identified in the confidence intervals of nine QTLs impacting shoot dry weight at LK, and 22 of these showed variations in coding sequences and/or exhibited significant differences in mRNA abundances in roots at LK between the two parental lines. This study provided insights to the genetic basis of KUE in B. napus, which will accelerate the breeding of K-efficient rapeseed cultivars by marker-assisted selection.",

keywords = "Brassica napus/genetics, INDEL Mutation, Ion Transport, Plant Proteins/genetics, Polymorphism, Single Nucleotide, Potassium/metabolism, Quantitative Trait Loci",

author = "Wei Wang and Jinsong Zou and White, {Philip J} and Guangda Ding and Yalin Li and Fangsen Xu and Lei Shi",

year = "2021",

month = mar,

doi = "10.1016/j.ygeno.2021.01.020",

language = "English",

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journal = "Genomics",

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TY - JOUR

T1 - Identification of QTLs associated with potassium use efficiency and underlying candidate genes by whole-genome resequencing of two parental lines in Brassica napus

AU - Wang, Wei

AU - Zou, Jinsong

AU - White, Philip J

AU - Ding, Guangda

AU - Li, Yalin

AU - Xu, Fangsen

AU - Shi, Lei

PY - 2021/3

Y1 - 2021/3

N2 - Breeding crops that acquire and/or utilize potassium (K) more effectively could reduce the use of K fertilizers. Sixteen traits affecting K use efficiency (KUE) at the seedling stage were investigated in a B. napus double haploid population grown at an optimal K supply (OK) and a low K supply (LK) in a hydroponic culture system. In total, 50 and 62 QTLs associated with these traits were identified at OK and LK, respectively. A total of 25 orthologues of 23 Arabidopsis genes regulating K transport were identified in the confidence intervals of nine QTLs impacting shoot dry weight at LK, and 22 of these showed variations in coding sequences and/or exhibited significant differences in mRNA abundances in roots at LK between the two parental lines. This study provided insights to the genetic basis of KUE in B. napus, which will accelerate the breeding of K-efficient rapeseed cultivars by marker-assisted selection.

AB - Breeding crops that acquire and/or utilize potassium (K) more effectively could reduce the use of K fertilizers. Sixteen traits affecting K use efficiency (KUE) at the seedling stage were investigated in a B. napus double haploid population grown at an optimal K supply (OK) and a low K supply (LK) in a hydroponic culture system. In total, 50 and 62 QTLs associated with these traits were identified at OK and LK, respectively. A total of 25 orthologues of 23 Arabidopsis genes regulating K transport were identified in the confidence intervals of nine QTLs impacting shoot dry weight at LK, and 22 of these showed variations in coding sequences and/or exhibited significant differences in mRNA abundances in roots at LK between the two parental lines. This study provided insights to the genetic basis of KUE in B. napus, which will accelerate the breeding of K-efficient rapeseed cultivars by marker-assisted selection.

KW - Brassica napus/genetics

KW - INDEL Mutation

KW - Ion Transport

KW - Plant Proteins/genetics

KW - Polymorphism, Single Nucleotide

KW - Potassium/metabolism

KW - Quantitative Trait Loci

U2 - 10.1016/j.ygeno.2021.01.020

DO - 10.1016/j.ygeno.2021.01.020

M3 - Article

C2 - 33516850

SN - 1471-2164

VL - 113

SP - 755

EP - 768

JO - Genomics

JF - Genomics

IS - 2

ER -

Identification of QTLs associated with potassium use efficiency and underlying candidate genes by whole-genome resequencing of two parental lines in Brassica napus

Abstract

Keywords

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