Identification of RAPD markers linked to genetic factors controlling the milling energy requirement of barley

K. J. Chalmers, U. M. Barua, C. A. Hackett, Thomas, R. Waugh, W. Powell (Lead / Corresponding author)

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Doubled haploid (DH) populations of barley have been used in combination with PCR-based polymorphic-assay procedures to identify molecular markers linked to genes controlling the milling energy requirement of the grain. Milling energy (ME) is a quantitative trait and locating individual quantitative trait loci (QTLs) involved the construction of bulks by combining DNA from DH families representing the extreme members of the distribution for ME. In addition, the individuals had alternative alleles at the Rrn2 locus that has previously been shown to be linked to an ME QTL. The DNA bulks were screened with Randomly Amplified Polymorphic DNA (RAPD) markers and polymorphic amplification products tested for linkage to genes influencing the expression of ME in a DH population. Several markers were identified which are linked to a QTL controlling ME and the recombination fraction determined by maximum likelihood procedures. The results indicate that DHs in combination with RAPDs and bulked segregant analysis provide an efficient method for locating QTLs in barely. Furthermore, this approach is applicable to mapping other QTLs in a range of organisms from which DH or recombinant inbred lines can be extracted.

Original languageEnglish
Pages (from-to)314-320
Number of pages7
JournalTheoretical and Applied Genetics: International Journal of Plant Breeding Research
Volume87
Issue number3
DOIs
Publication statusPublished - Nov 1993

Keywords

  • Barley
  • Bulked segregant analysis
  • DHs
  • QTLs
  • RAPDs

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