Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)

Miles Armstrong, Ingo Hein (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Diagnostic Resistance gene enrichment Sequencing (dRenSeq) is a method for tracking and verifying the integrity of multiple functional NLR genes in complex genetic backgrounds. Traditionally, these tasks have been addressed by laborious and time-consuming PCR-based approaches or, where cognate avirulence genes are available, effector recognition studies. Both these approaches are superseded by dRenSeq in terms of cost-effectiveness, efficiency, and accuracy.

Original languageEnglish
Title of host publicationSolanum tuberosum
Subtitle of host publicationMethods and Protocols
EditorsDavid Dobnik, Kristina Gruden, Živa Ramšak, Anna Coll
Place of PublicationNew York
PublisherHumana Press
Pages213-219
Number of pages7
Edition1
ISBN (Electronic)9781071616093
ISBN (Print)9781071616086 (hbk), 9781071616116 (pbk)
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2354
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Coverage calculation
  • Enrichment sequencing
  • Illumina
  • Plant resistance
  • Read mapping

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