Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)

Miles Armstrong; Ingo Hein

doi:10.1007/978-1-0716-1609-3_10

Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)

Miles Armstrong
, Ingo Hein (Lead / Corresponding author)

Plant Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

Diagnostic Resistance gene enrichment Sequencing (dRenSeq) is a method for tracking and verifying the integrity of multiple functional NLR genes in complex genetic backgrounds. Traditionally, these tasks have been addressed by laborious and time-consuming PCR-based approaches or, where cognate avirulence genes are available, effector recognition studies. Both these approaches are superseded by dRenSeq in terms of cost-effectiveness, efficiency, and accuracy.

Original language	English
Title of host publication	Solanum tuberosum
Subtitle of host publication	Methods and Protocols
Editors	David Dobnik, Kristina Gruden, Živa Ramšak, Anna Coll
Place of Publication	New York
Publisher	Humana Press
Pages	213-219
Number of pages	7
Edition	1
ISBN (Electronic)	9781071616093
ISBN (Print)	9781071616086 (hbk), 9781071616116 (pbk)
DOIs	https://doi.org/10.1007/978-1-0716-1609-3_10
Publication status	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2354
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Coverage calculation
Enrichment sequencing
Illumina
Plant resistance
Read mapping

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-1609-3_10

Cite this

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title = "Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)",

abstract = "Diagnostic Resistance gene enrichment Sequencing (dRenSeq) is a method for tracking and verifying the integrity of multiple functional NLR genes in complex genetic backgrounds. Traditionally, these tasks have been addressed by laborious and time-consuming PCR-based approaches or, where cognate avirulence genes are available, effector recognition studies. Both these approaches are superseded by dRenSeq in terms of cost-effectiveness, efficiency, and accuracy.",

keywords = "Coverage calculation, Enrichment sequencing, Illumina, Plant resistance, Read mapping",

author = "Miles Armstrong and Ingo Hein",

note = "Funding: The Scottish Government's Rural and Environment Science and Analytical Services (RESAS) through WP2 and UKRI, BBSRC funding through grants BB/L008025/1 and BB/S015663/1. {\textcopyright} 2021. Springer Science+Business Media, LLC, part of Springer Nature.",

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publisher = "Humana Press",

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Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq). / Armstrong, Miles; Hein, Ingo (Lead / Corresponding author).
Solanum tuberosum: Methods and Protocols. ed. / David Dobnik; Kristina Gruden; Živa Ramšak; Anna Coll. 1. ed. New York: Humana Press, 2021. p. 213-219 (Methods in Molecular Biology; Vol. 2354).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)

AU - Armstrong, Miles

AU - Hein, Ingo

N1 - Funding: The Scottish Government's Rural and Environment Science and Analytical Services (RESAS) through WP2 and UKRI, BBSRC funding through grants BB/L008025/1 and BB/S015663/1. © 2021. Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2021

Y1 - 2021

N2 - Diagnostic Resistance gene enrichment Sequencing (dRenSeq) is a method for tracking and verifying the integrity of multiple functional NLR genes in complex genetic backgrounds. Traditionally, these tasks have been addressed by laborious and time-consuming PCR-based approaches or, where cognate avirulence genes are available, effector recognition studies. Both these approaches are superseded by dRenSeq in terms of cost-effectiveness, efficiency, and accuracy.

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KW - Coverage calculation

KW - Enrichment sequencing

KW - Illumina

KW - Plant resistance

KW - Read mapping

UR - https://www.scopus.com/pages/publications/85114203092

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DO - 10.1007/978-1-0716-1609-3_10

M3 - Chapter (peer-reviewed)

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SN - 9781071616116 (pbk)

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PB - Humana Press

CY - New York

ER -

Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Studying Co Evolution in Agriculture to Inform NLR Deployment (Industrial Partnership Award - IPA)

Cite this

Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Studying Co Evolution in Agriculture to Inform NLR Deployment (Industrial Partnership Award - IPA)

Cite this