Identification of the antigen recognized by the monoclonal antibody BU31 as lamins A and C

Philip J. Coates, R. Carl Hobbs, John Crocker, David C. Rowlands, Paul Murray, Roy Quinlan, Peter A. Hall

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    The murine monoclonal antibody BU31 binds to the nuclear membrane of many cell types. The expression of the BU31 antigen has previously been shown to have an inverse correlation with the proliferative index in lung tumours, defined by Ki67 staining. The distribution of BU31-positive cells is now shown to parallel the distribution of non-dividing cells in a range of normal human and rat tissues, although neuroendocrine cells and germ cells in the testis show no reactivity. Cells grown in culture and induced to undergo growth arrest show a higher level of labelling with BU31 than their proliferating counterparts. Confocal laser scanning microscopy reveals that the BU31 antigen is distributed predominantly along the nuclear lamina, with occasional internal foci. This distribution is very similar to that of the nuclear membrane proteins lamin A and lamin C, suggesting that the BU31 antigen and lamins A and C could be one and the same. Immunoblotting using recombinant lamin proteins confirmed this proposal. Moreover, a monoclonal antibody to the non-proliferation-associated antigen, statin, also recognizes lamins A and C. These data indicate that the demonstration of lamins A and C can be used to provide information on the proliferative activity of normal and neoplastic tissues. These data also suggest a role for nuclear lamins A and C during cellular quiescence, possibly through the reorganization and maintenance of nuclear structure, or more directly through interactions with the retinoblastoma gene product or related proteins.
    Original languageEnglish
    Pages (from-to)21-29
    Number of pages9
    JournalJournal of Pathology
    Volume178
    Issue number1
    DOIs
    Publication statusPublished - 1996

    Fingerprint

    Lamin Type A
    Monoclonal Antibodies
    Antigens
    Nuclear Envelope
    Nuclear Lamina
    Lamins
    Retinoblastoma Genes
    Hydroxymethylglutaryl-CoA Reductase Inhibitors
    Neuroendocrine Cells
    Nuclear Proteins
    Recombinant Proteins
    Immunoblotting
    Germ Cells
    Confocal Microscopy
    Testis
    Membrane Proteins
    Reference Values
    Maintenance
    Staining and Labeling
    Lung

    Cite this

    Coates, Philip J. ; Hobbs, R. Carl ; Crocker, John ; Rowlands, David C. ; Murray, Paul ; Quinlan, Roy ; Hall, Peter A. / Identification of the antigen recognized by the monoclonal antibody BU31 as lamins A and C. In: Journal of Pathology. 1996 ; Vol. 178, No. 1. pp. 21-29.
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    abstract = "The murine monoclonal antibody BU31 binds to the nuclear membrane of many cell types. The expression of the BU31 antigen has previously been shown to have an inverse correlation with the proliferative index in lung tumours, defined by Ki67 staining. The distribution of BU31-positive cells is now shown to parallel the distribution of non-dividing cells in a range of normal human and rat tissues, although neuroendocrine cells and germ cells in the testis show no reactivity. Cells grown in culture and induced to undergo growth arrest show a higher level of labelling with BU31 than their proliferating counterparts. Confocal laser scanning microscopy reveals that the BU31 antigen is distributed predominantly along the nuclear lamina, with occasional internal foci. This distribution is very similar to that of the nuclear membrane proteins lamin A and lamin C, suggesting that the BU31 antigen and lamins A and C could be one and the same. Immunoblotting using recombinant lamin proteins confirmed this proposal. Moreover, a monoclonal antibody to the non-proliferation-associated antigen, statin, also recognizes lamins A and C. These data indicate that the demonstration of lamins A and C can be used to provide information on the proliferative activity of normal and neoplastic tissues. These data also suggest a role for nuclear lamins A and C during cellular quiescence, possibly through the reorganization and maintenance of nuclear structure, or more directly through interactions with the retinoblastoma gene product or related proteins.",
    author = "Coates, {Philip J.} and Hobbs, {R. Carl} and John Crocker and Rowlands, {David C.} and Paul Murray and Roy Quinlan and Hall, {Peter A.}",
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    Identification of the antigen recognized by the monoclonal antibody BU31 as lamins A and C. / Coates, Philip J.; Hobbs, R. Carl; Crocker, John; Rowlands, David C.; Murray, Paul; Quinlan, Roy; Hall, Peter A.

    In: Journal of Pathology, Vol. 178, No. 1, 1996, p. 21-29.

    Research output: Contribution to journalArticle

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    AU - Coates, Philip J.

    AU - Hobbs, R. Carl

    AU - Crocker, John

    AU - Rowlands, David C.

    AU - Murray, Paul

    AU - Quinlan, Roy

    AU - Hall, Peter A.

    PY - 1996

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    AB - The murine monoclonal antibody BU31 binds to the nuclear membrane of many cell types. The expression of the BU31 antigen has previously been shown to have an inverse correlation with the proliferative index in lung tumours, defined by Ki67 staining. The distribution of BU31-positive cells is now shown to parallel the distribution of non-dividing cells in a range of normal human and rat tissues, although neuroendocrine cells and germ cells in the testis show no reactivity. Cells grown in culture and induced to undergo growth arrest show a higher level of labelling with BU31 than their proliferating counterparts. Confocal laser scanning microscopy reveals that the BU31 antigen is distributed predominantly along the nuclear lamina, with occasional internal foci. This distribution is very similar to that of the nuclear membrane proteins lamin A and lamin C, suggesting that the BU31 antigen and lamins A and C could be one and the same. Immunoblotting using recombinant lamin proteins confirmed this proposal. Moreover, a monoclonal antibody to the non-proliferation-associated antigen, statin, also recognizes lamins A and C. These data indicate that the demonstration of lamins A and C can be used to provide information on the proliferative activity of normal and neoplastic tissues. These data also suggest a role for nuclear lamins A and C during cellular quiescence, possibly through the reorganization and maintenance of nuclear structure, or more directly through interactions with the retinoblastoma gene product or related proteins.

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