Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Alastair Aitken, Philip Cohen, Sitthivet Santikarn, Dudley H. Williams, A. Graham Calder, Alistair Smith, Claude B. Klee

    Research output: Contribution to journalArticle

    167 Citations (Scopus)

    Abstract

    The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-GlyAsnGluAla-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

    Original languageEnglish
    Pages (from-to)314-318
    Number of pages5
    JournalFEBS Letters
    Volume150
    Issue number2
    DOIs
    Publication statusPublished - 27 Dec 1982

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    Calcineurin
    Myristic Acid
    Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
    Fast Atom Bombardment Mass Spectrometry
    Gas chromatography
    Gas Chromatography-Mass Spectrometry
    Mass spectrometry
    Proteins
    Fatty Acids
    High Pressure Liquid Chromatography
    Atoms
    Peptides
    acetonitrile
    calcineurin phosphatase

    Keywords

    • Ca
    • Calmodulin
    • Fatty acids
    • High-performance liquid chromatography
    • Mass spectrometry
    • Protein phosphatase

    Cite this

    Aitken, A., Cohen, P., Santikarn, S., Williams, D. H., Calder, A. G., Smith, A., & Klee, C. B. (1982). Identification of the NH2-terminal blocking group of calcineurin B as myristic acid. FEBS Letters, 150(2), 314-318. https://doi.org/10.1016/0014-5793(82)80759-X
    Aitken, Alastair ; Cohen, Philip ; Santikarn, Sitthivet ; Williams, Dudley H. ; Calder, A. Graham ; Smith, Alistair ; Klee, Claude B. / Identification of the NH2-terminal blocking group of calcineurin B as myristic acid. In: FEBS Letters. 1982 ; Vol. 150, No. 2. pp. 314-318.
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    abstract = "The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-GlyAsnGluAla-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57{\%} acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.",
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    Aitken, A, Cohen, P, Santikarn, S, Williams, DH, Calder, AG, Smith, A & Klee, CB 1982, 'Identification of the NH2-terminal blocking group of calcineurin B as myristic acid', FEBS Letters, vol. 150, no. 2, pp. 314-318. https://doi.org/10.1016/0014-5793(82)80759-X

    Identification of the NH2-terminal blocking group of calcineurin B as myristic acid. / Aitken, Alastair; Cohen, Philip; Santikarn, Sitthivet; Williams, Dudley H.; Calder, A. Graham; Smith, Alistair; Klee, Claude B.

    In: FEBS Letters, Vol. 150, No. 2, 27.12.1982, p. 314-318.

    Research output: Contribution to journalArticle

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    AU - Aitken, Alastair

    AU - Cohen, Philip

    AU - Santikarn, Sitthivet

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    AU - Calder, A. Graham

    AU - Smith, Alistair

    AU - Klee, Claude B.

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    AB - The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-GlyAsnGluAla-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

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