Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Alastair Aitken, Philip Cohen, Sitthivet Santikarn, Dudley H. Williams, A. Graham Calder, Alistair Smith, Claude B. Klee

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    Abstract

    The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-GlyAsnGluAla-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

    Original languageEnglish
    Pages (from-to)314-318
    Number of pages5
    JournalFEBS Letters
    Volume150
    Issue number2
    DOIs
    Publication statusPublished - 27 Dec 1982

    Keywords

    • Ca
    • Calmodulin
    • Fatty acids
    • High-performance liquid chromatography
    • Mass spectrometry
    • Protein phosphatase

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