Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Alastair Aitken, Philip Cohen, Sitthivet Santikarn, Dudley H. Williams, A. Graham Calder, Alistair Smith, Claude B. Klee

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    Abstract

    The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-GlyAsnGluAla-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

    Original languageEnglish
    Pages (from-to)314-318
    Number of pages5
    JournalFEBS Letters
    Volume150
    Issue number2
    DOIs
    Publication statusPublished - 27 Dec 1982

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    Keywords

    • Ca
    • Calmodulin
    • Fatty acids
    • High-performance liquid chromatography
    • Mass spectrometry
    • Protein phosphatase

    Cite this

    Aitken, A., Cohen, P., Santikarn, S., Williams, D. H., Calder, A. G., Smith, A., & Klee, C. B. (1982). Identification of the NH2-terminal blocking group of calcineurin B as myristic acid. FEBS Letters, 150(2), 314-318. https://doi.org/10.1016/0014-5793(82)80759-X