Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Alastair Aitken; Philip Cohen; Sitthivet Santikarn; Dudley H. Williams; A. Graham Calder; Alistair Smith; Claude B. Klee

doi:10.1016/0014-5793(82)80759-X

Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Alastair Aitken
, Philip Cohen
, Sitthivet Santikarn
, Dudley H. Williams
, A. Graham Calder
, Alistair Smith
, Claude B. Klee

Research output: Contribution to journal › Article › peer-review

210 Citations (Scopus)

Abstract

The NH₂-terminal blocking group of the Ca²⁺-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-GlyAsnGluAla-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

Original language	English
Pages (from-to)	314-318
Number of pages	5
Journal	FEBS Letters
Volume	150
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(82)80759-X
Publication status	Published - 27 Dec 1982

Keywords

Ca
Calmodulin
Fatty acids
High-performance liquid chromatography
Mass spectrometry
Protein phosphatase

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Identification of the NH2-terminal blocking group of calcineurin B as myristic acid",

abstract = "The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-GlyAsnGluAla-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57\% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.",

keywords = "Ca, Calmodulin, Fatty acids, High-performance liquid chromatography, Mass spectrometry, Protein phosphatase",

author = "Alastair Aitken and Philip Cohen and Sitthivet Santikarn and Williams, \{Dudley H.\} and Calder, \{A. Graham\} and Alistair Smith and Klee, \{Claude B.\}",

year = "1982",

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day = "27",

doi = "10.1016/0014-5793(82)80759-X",

language = "English",

volume = "150",

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journal = "FEBS Letters",

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T1 - Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

AU - Aitken, Alastair

AU - Cohen, Philip

AU - Santikarn, Sitthivet

AU - Williams, Dudley H.

AU - Calder, A. Graham

AU - Smith, Alistair

AU - Klee, Claude B.

PY - 1982/12/27

Y1 - 1982/12/27

N2 - The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-GlyAsnGluAla-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

AB - The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-GlyAsnGluAla-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

KW - Ca

KW - Calmodulin

KW - Fatty acids

KW - High-performance liquid chromatography

KW - Mass spectrometry

KW - Protein phosphatase

UR - https://www.scopus.com/pages/publications/0020492523

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DO - 10.1016/0014-5793(82)80759-X

M3 - Article

C2 - 7160476

AN - SCOPUS:0020492523

SN - 0014-5793

VL - 150

SP - 314

EP - 318

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Abstract

Keywords

ASJC Scopus subject areas

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