Identifying cytokine signaling signatures in primary human Th-1 cells by phospho-proteomics analysis

Jonathan Martinez-Fabregas (Lead / Corresponding author), Elizabeth Pohler, Ignacio Moraga (Lead / Corresponding author)

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Abstract

Stable isotope labeling by amino acid-based high-resolution phosphoproteomics is a powerful technique that allows for direct comparison of cells stimulated under different experimental conditions. This feature makes it the ideal methodology to identify cytokine signaling networks. Here, we present an optimized protocol for the isolation and identification of phosphopeptides from IL-6-stimulated primary human Th-1 cells. For complete details on the use and execution of this protocol, please refer to Martinez-Fabregas et al. (2020).

Original languageEnglish
Article number100417
Number of pages14
JournalSTAR Protocols
Volume2
Issue number2
Early online date31 Mar 2021
DOIs
Publication statusE-pub ahead of print - 31 Mar 2021

Keywords

  • Cell isolation
  • Immunology
  • Protein Biochemistry
  • Proteomics
  • Signal Transduction

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