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Abstract
Stable isotope labeling by amino acid-based high-resolution phosphoproteomics is a powerful technique that allows for direct comparison of cells stimulated under different experimental conditions. This feature makes it the ideal methodology to identify cytokine signaling networks. Here, we present an optimized protocol for the isolation and identification of phosphopeptides from IL-6-stimulated primary human Th-1 cells. For complete details on the use and execution of this protocol, please refer to Martinez-Fabregas et al. (2020).
Original language | English |
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Article number | 100417 |
Number of pages | 14 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 2 |
Early online date | 31 Mar 2021 |
DOIs | |
Publication status | Published - 18 Jun 2021 |
Keywords
- Cell isolation
- Immunology
- Protein Biochemistry
- Proteomics
- Signal Transduction
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology
- General Neuroscience
- General Immunology and Microbiology
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Dive into the research topics of 'Identifying cytokine signaling signatures in primary human Th-1 cells by phospho-proteomics analysis'. Together they form a unique fingerprint.Projects
- 1 Finished
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Mapping Cytokine Signalling Networks using Engineered Surrogate Ligands (Sir Henry Dale Fellowship)
Crocker, P. (Investigator) & Moraga Gonzalez, I. (Investigator)
1/09/16 → 31/08/22
Project: Research