desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 in barley

Isabelle Colas, Abdellah Barakate (Lead / Corresponding author), Malcolm Macaulay, Miriam Schreiber, Jennifer Stephens, Sebastian Vivera, Claire Halpin, Robbie Waugh (Lead / Corresponding author), Luke Ramsay (Lead / Corresponding author)

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Abstract

Despite conservation of the process of meiosis, recombination landscapes vary between species, with large genome grasses such as barley (Hordeum vulgare L.) exhibiting a pattern of recombination that is very heavily skewed to the ends of chromosomes. We have been using a collection of semi-sterile desynaptic meiotic mutant lines to help elucidate how recombination is controlled in barley and the role of the corresponding wild-type (WT) meiotic genes within this process. Here we applied a combination of genetic segregation analysis, cytogenetics, and immunocytology to genetically map and characterize the meiotic mutant desynaptic5 (des5). We identified an exonic insertion in the positional candidate ortholog of Disrupted Meiotic cDNA 1 (HvDMC1) on chromosome 5H of des5. des5 exhibits a severe meiotic phenotype with disturbed synapsis, reduced crossovers, and chromosome mis-segregation. The meiotic phenotype and reduced fertility of des5 is similarly observed in Hvdmc1RNAi transgenic plants and HvDMC1p:GusPlus reporter lines show DMC1 expression specifically in the developing inflorescence. The des5 mutation maintains the reading frame of the gene and exhibits semi-dominance with respect to recombination in the heterozygote indicating the value of non-knockout mutations for dissection of the control of recombination in the early stages of meiosis.

Original languageEnglish
Pages (from-to)2683-2698
Number of pages16
JournalJournal of Experimental Botany
Volume70
Issue number10
Early online date27 Apr 2019
DOIs
Publication statusPublished - 1 May 2019

Fingerprint

Hordeum
Genetic Recombination
Complementary DNA
barley
chromosomes
mutation
meiosis
Mutation
incomplete dominance
Meiosis
phenotype
mutants
cytogenetic analysis
Hordeum vulgare
Chromosomes
transgenic plants
heterozygosity
Chromosome Pairing
Phenotype
inflorescences

Keywords

  • Barley
  • DMC1
  • RNAi
  • crossover
  • desynaptic
  • immunocytology
  • meiosis
  • recombination

Cite this

Colas, I., Barakate, A., Macaulay, M., Schreiber, M., Stephens, J., Vivera, S., ... Ramsay, L. (2019). desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 in barley. Journal of Experimental Botany, 70(10), 2683-2698. https://doi.org/10.1093/jxb/erz080
Colas, Isabelle ; Barakate, Abdellah ; Macaulay, Malcolm ; Schreiber, Miriam ; Stephens, Jennifer ; Vivera, Sebastian ; Halpin, Claire ; Waugh, Robbie ; Ramsay, Luke. / desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 in barley. In: Journal of Experimental Botany. 2019 ; Vol. 70, No. 10. pp. 2683-2698.
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Colas, I, Barakate, A, Macaulay, M, Schreiber, M, Stephens, J, Vivera, S, Halpin, C, Waugh, R & Ramsay, L 2019, 'desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 in barley', Journal of Experimental Botany, vol. 70, no. 10, pp. 2683-2698. https://doi.org/10.1093/jxb/erz080

desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 in barley. / Colas, Isabelle; Barakate, Abdellah (Lead / Corresponding author); Macaulay, Malcolm; Schreiber, Miriam; Stephens, Jennifer; Vivera, Sebastian; Halpin, Claire; Waugh, Robbie (Lead / Corresponding author); Ramsay, Luke (Lead / Corresponding author).

In: Journal of Experimental Botany, Vol. 70, No. 10, 01.05.2019, p. 2683-2698.

Research output: Contribution to journalArticle

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T1 - desynaptic5 carries a spontaneous semi-dominant mutation affecting Disrupted Meiotic cDNA 1 in barley

AU - Colas, Isabelle

AU - Barakate, Abdellah

AU - Macaulay, Malcolm

AU - Schreiber, Miriam

AU - Stephens, Jennifer

AU - Vivera, Sebastian

AU - Halpin, Claire

AU - Waugh, Robbie

AU - Ramsay, Luke

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Y1 - 2019/5/1

N2 - Despite conservation of the process of meiosis, recombination landscapes vary between species, with large genome grasses such as barley (Hordeum vulgare L.) exhibiting a pattern of recombination that is very heavily skewed to the ends of chromosomes. We have been using a collection of semi-sterile desynaptic meiotic mutant lines to help elucidate how recombination is controlled in barley and the role of the corresponding wild-type (WT) meiotic genes within this process. Here we applied a combination of genetic segregation analysis, cytogenetics, and immunocytology to genetically map and characterize the meiotic mutant desynaptic5 (des5). We identified an exonic insertion in the positional candidate ortholog of Disrupted Meiotic cDNA 1 (HvDMC1) on chromosome 5H of des5. des5 exhibits a severe meiotic phenotype with disturbed synapsis, reduced crossovers, and chromosome mis-segregation. The meiotic phenotype and reduced fertility of des5 is similarly observed in Hvdmc1RNAi transgenic plants and HvDMC1p:GusPlus reporter lines show DMC1 expression specifically in the developing inflorescence. The des5 mutation maintains the reading frame of the gene and exhibits semi-dominance with respect to recombination in the heterozygote indicating the value of non-knockout mutations for dissection of the control of recombination in the early stages of meiosis.

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KW - recombination

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