E unum pluribus: multiple proteins from a self-processing polyprotein

Pablo de Felipe, Garry A. Luke, Lorraine E. Hughes, David Gani, Claire Halpin, Martin D. Ryan (Lead / Corresponding author)

    Research output: Contribution to journalReview articlepeer-review

    278 Citations (Scopus)


    Many applications of genetic engineering require transformation with multiple (trans)genes, although to achieve these using conventional techniques can be challenging. The 2A oligopeptide is emerging as a highly effective new tool for the facile co-expression of multiple proteins in a single transformation step, whereby a gene encoding multiple proteins, linked by 2A sequences, is transcribed from a single promoter. The polyprotein self-processes co-translationally such that each constituent protein is generated as a discrete translation product. 2A functions in all the eukaryotic systems tested to date and has already been applied, with great success, to a broad range of biotechnological applications: from plant metabolome engineering to the expression of T-cell receptor complexes, monoclonal antibodies or heterodimeric cytokines in animals.

    Original languageEnglish
    Pages (from-to)68-75
    Number of pages8
    JournalTrends in Biotechnology
    Issue number2
    Publication statusPublished - Feb 2006

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering


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