E unum pluribus: multiple proteins from a self-processing polyprotein

Pablo de Felipe, Garry A. Luke, Lorraine E. Hughes, David Gani, Claire Halpin, Martin D. Ryan (Lead / Corresponding author)

    Research output: Contribution to journalReview articlepeer-review

    266 Citations (Scopus)

    Abstract

    Many applications of genetic engineering require transformation with multiple (trans)genes, although to achieve these using conventional techniques can be challenging. The 2A oligopeptide is emerging as a highly effective new tool for the facile co-expression of multiple proteins in a single transformation step, whereby a gene encoding multiple proteins, linked by 2A sequences, is transcribed from a single promoter. The polyprotein self-processes co-translationally such that each constituent protein is generated as a discrete translation product. 2A functions in all the eukaryotic systems tested to date and has already been applied, with great success, to a broad range of biotechnological applications: from plant metabolome engineering to the expression of T-cell receptor complexes, monoclonal antibodies or heterodimeric cytokines in animals.

    Original languageEnglish
    Pages (from-to)68-75
    Number of pages8
    JournalTrends in Biotechnology
    Volume24
    Issue number2
    DOIs
    Publication statusPublished - Feb 2006

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