Euglena gracilis Genome and Transcriptome: Organelles, Nuclear Genome Assembly Strategies and Initial Features

ThankGod Echezona Ebenezer, Mark Carrington, Michael Lebert, Steven Kelly (Lead / Corresponding author), Mark C. Field (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

19 Citations (Scopus)

Abstract

Euglena gracilis is a major component of the aquatic ecosystem and together with closely related species, is ubiquitous worldwide. Euglenoids are an important group of protists, possessing a secondarily acquired plastid and are relatives to the Kinetoplastidae, which themselves have global impact as disease agents. To understand the biology of E. gracilis, as well as to provide further insight into the evolution and origins of the Kinetoplastidae, we embarked on sequencing the nuclear genome; the plastid and mitochondrial genomes are already in the public domain. Earlier studies suggested an extensive nuclear DNA content, with likely a high degree of repetitive sequence, together with significant extrachromosomal elements. To produce a list of coding sequences we have combined transcriptome data from both published and new sources, as well as embarked on de novo sequencing using a combination of 454, Illumina paired end libraries and long PacBio reads. Preliminary analysis suggests a surprisingly large genome approaching 2 Gbp, with a highly fragmented architecture and extensive repeat composition. Over 80% of the RNAseq reads from E. gracilis maps to the assembled genome sequence, which is comparable with the well assembled genomes of T. brucei and T. cruzi. In order to achieve this level of assembly we employed multiple informatics pipelines, which are discussed here. Finally, as a preliminary view of the genome architecture, we discuss the tubulin and calmodulin genes, which highlight potential novel splicing mechanisms.

Original languageEnglish
Title of host publicationEuglena
Subtitle of host publicationBiochemistry, Cell and Molecular Biology
EditorsSteven D. Schwartzbach, Shigeru Shigeoka
Place of PublicationSwitzerland
PublisherSpringer International Publishing
Pages125-140
Number of pages16
ISBN (Electronic)9783319549101
ISBN (Print)9783319549088
DOIs
Publication statusPublished - 21 Apr 2017

Publication series

NameAdvances in Experimental Medicine and Biology
PublisherSpringer International Publishing
Volume979
ISSN (Print)0065-2598

Keywords

  • Euglena
  • Next generation sequencing
  • Genome assembly
  • Tubulin
  • Genome architecture
  • Splicing
  • Secondary endosymbiosis

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    Ebenezer, T. E., Carrington, M., Lebert, M., Kelly, S., & Field, M. C. (2017). Euglena gracilis Genome and Transcriptome: Organelles, Nuclear Genome Assembly Strategies and Initial Features. In S. D. Schwartzbach, & S. Shigeoka (Eds.), Euglena: Biochemistry, Cell and Molecular Biology (pp. 125-140). (Advances in Experimental Medicine and Biology; Vol. 979). Springer International Publishing. https://doi.org/10.1007/978-3-319-54910-1_7