In vitro reconstitution defines the minimal requirements for Cdc48-dependent disassembly of the CMG helicase in budding yeast

Progya Mukherjee, Karim Labib (Lead / Corresponding author)

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Abstract

Disassembly of the replisome is the final step of chromosome duplication in eukaryotes. In budding yeast and metazoa, cullin ubiquitin ligases are required to ubiquitylate the Cdc45-MCM-GINS (CMG) helicase that lies at the heart of the replisome, leading to a disassembly reaction that is dependent upon the ATPase known as Cdc48 or p97. Here, we describe the reconstitution of replisome disassembly, using a purified complex of the budding yeast replisome in association with the cullin ligase SCF Dia2. Upon addition of E1 and E2 enzymes, together with ubiquitin and ATP, the CMG helicase is ubiquitylated on its Mcm7 subunit. Subsequent addition of Cdc48, together with its cofactors Ufd1-Npl4, drives efficient disassembly of ubiquitylated CMG, thereby recapitulating the steps of replisome disassembly that are observed in vivo. Our findings define the minimal requirements for disassembly of the eukaryotic replisome and provide a model system for studying the disassembly of protein complexes by Cdc48-Ufd1-Npl4.

Original languageEnglish
Pages (from-to)2777-2783.e4
Number of pages11
JournalCell Reports
Volume28
Issue number11
Early online date10 Sep 2019
DOIs
Publication statusPublished - 10 Sep 2019

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Keywords

  • ATPase
  • CMG helicase
  • Cdc48
  • DNA replication
  • Mcm7
  • SCF
  • Ufd1-Npl4
  • p97
  • ubiquitylation

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