Projects per year
Abstract
The rapid formation and activation of the NLRP3 inflammasome is induced by co-stimulation with LPS and nigericin. It requires the LPS-stimulated activation of IKKβ, which exerts its effects independently of de novo gene transcription, protein translation and other protein kinases activated by IKKβ. IKKβ is not required for the nigericin-induced dispersion of the trans-Golgi network (TGN), but to bring NLRP3 in proximity with TGN38. The nigericin-induced dispersion of the Golgi is enhanced by co-stimulation with LPS, and this enhancement is IKKβ-dependent. Prolonged stimulation with LPS to increase the expression of NLRP3, followed by stimulation with nigericin, produced larger TGN38-positive puncta, and the ensuing activation of the NLRP3 inflammasome was also suppressed by IKKβ inhibitors added prior to stimulation with nigericin. IKKβ therefore has a key role in recruiting NLRP3 to the dispersed TGN, leading to the formation and activation of the NLRP3 inflammasome.
Original language | English |
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Article number | e50743 |
Number of pages | 14 |
Journal | EMBO Reports |
Volume | 22 |
Issue number | 10 |
Early online date | 17 Aug 2021 |
DOIs | |
Publication status | Published - 5 Oct 2021 |
Keywords
- inflammasome
- innate immunity
- kinases
- toll-like receptors
- trans-golgi network
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics
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- 3 Finished
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The Molecular Mechanisms by which TRAF6 Regulates the Immune System
Cohen, P. (Investigator)
1/04/18 → 31/03/24
Project: Research
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Elucidation of Mechanisms that Restrict Activation of the Innate Immune System to Prevent Inflammatory and Autoimmune Diseases
Arthur, S. (Investigator) & Cohen, P. (Investigator)
1/04/18 → 31/03/23
Project: Research
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Dissecting how the NLRP3 Inflammasome is Activated to Treat Chronic Inflammatory Diseases
Cohen, P. (Investigator) & Nanda, S. (Investigator)
15/10/16 → 14/10/17
Project: Research