Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue

P. C. Boevink, P. R. Birch, S. C. Whisson

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    6 Citations (Scopus)

    Abstract

    Assays to determine the role of pathogen effectors within an infected plant cell are yielding valuable information about which host processes are targeted to allow successful pathogen colonization. However, this does not necessarily inform on the cellular location of these interactions, or if these effector-virulence target interactions occur only in the presence of the pathogen. Here, we describe techniques to allow the subcellular localization of pathogen effectors inside infected plant cells or tissues, based largely on infiltration of plant tissue by Agrobacterium tumefaciens and its delivery of DNA encoding fluorescent protein-tagged effectors, and subsequent confocal microscopy.
    Original languageEnglish
    Title of host publicationPlant immunity
    Subtitle of host publicationmethods and protocols
    EditorsJohn D. McDowell
    Place of PublicationNew York
    PublisherHumana Press
    Pages195-209
    Number of pages15
    ISBN (Electronic)9781617379987
    ISBN (Print)9781617379970
    DOIs
    Publication statusPublished - 2011

    Publication series

    NameMethods in Molecular Biology
    PublisherHumana Press
    Volume712
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

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  • Cite this

    Boevink, P. C., Birch, P. R., & Whisson, S. C. (2011). Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue. In J. D. McDowell (Ed.), Plant immunity: methods and protocols (pp. 195-209). (Methods in Molecular Biology; Vol. 712). Humana Press. https://doi.org/10.1007/978-1-61737-998-7_16