Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue

P. C. Boevink; P. R. Birch; S. C. Whisson

doi:10.1007/978-1-61737-998-7_16

Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue

P. C. Boevink, P. R. Birch, S. C. Whisson

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed)

8 Citations (Scopus)

Abstract

Assays to determine the role of pathogen effectors within an infected plant cell are yielding valuable information about which host processes are targeted to allow successful pathogen colonization. However, this does not necessarily inform on the cellular location of these interactions, or if these effector-virulence target interactions occur only in the presence of the pathogen. Here, we describe techniques to allow the subcellular localization of pathogen effectors inside infected plant cells or tissues, based largely on infiltration of plant tissue by Agrobacterium tumefaciens and its delivery of DNA encoding fluorescent protein-tagged effectors, and subsequent confocal microscopy.

Original language	English
Title of host publication	Plant immunity
Subtitle of host publication	methods and protocols
Editors	John D. McDowell
Place of Publication	New York
Publisher	Humana Press
Pages	195-209
Number of pages	15
ISBN (Electronic)	9781617379987
ISBN (Print)	9781617379970
DOIs	https://doi.org/10.1007/978-1-61737-998-7_16
Publication status	Published - 2011

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	712
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Access to Document

10.1007/978-1-61737-998-7_16

Cite this

@inbook{edf3e40513934e5e9aa8309bcfa88438,

title = "Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue",

abstract = "Assays to determine the role of pathogen effectors within an infected plant cell are yielding valuable information about which host processes are targeted to allow successful pathogen colonization. However, this does not necessarily inform on the cellular location of these interactions, or if these effector-virulence target interactions occur only in the presence of the pathogen. Here, we describe techniques to allow the subcellular localization of pathogen effectors inside infected plant cells or tissues, based largely on infiltration of plant tissue by Agrobacterium tumefaciens and its delivery of DNA encoding fluorescent protein-tagged effectors, and subsequent confocal microscopy.",

author = "Boevink, {P. C.} and Birch, {P. R.} and Whisson, {S. C.}",

note = "MEDLINE{\textregistered} is the source for the citation and abstract of this record.",

year = "2011",

doi = "10.1007/978-1-61737-998-7_16",

language = "English",

isbn = "9781617379970",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "195--209",

editor = "McDowell, {John D.}",

booktitle = "Plant immunity",

address = "United States",

}

Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue. / Boevink, P. C.; Birch, P. R.; Whisson, S. C.
Plant immunity: methods and protocols. ed. / John D. McDowell. New York: Humana Press, 2011. p. 195-209 (Methods in Molecular Biology; Vol. 712).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed)

TY - CHAP

T1 - Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue

AU - Boevink, P. C.

AU - Birch, P. R.

AU - Whisson, S. C.

N1 - MEDLINE® is the source for the citation and abstract of this record.

PY - 2011

Y1 - 2011

N2 - Assays to determine the role of pathogen effectors within an infected plant cell are yielding valuable information about which host processes are targeted to allow successful pathogen colonization. However, this does not necessarily inform on the cellular location of these interactions, or if these effector-virulence target interactions occur only in the presence of the pathogen. Here, we describe techniques to allow the subcellular localization of pathogen effectors inside infected plant cells or tissues, based largely on infiltration of plant tissue by Agrobacterium tumefaciens and its delivery of DNA encoding fluorescent protein-tagged effectors, and subsequent confocal microscopy.

AB - Assays to determine the role of pathogen effectors within an infected plant cell are yielding valuable information about which host processes are targeted to allow successful pathogen colonization. However, this does not necessarily inform on the cellular location of these interactions, or if these effector-virulence target interactions occur only in the presence of the pathogen. Here, we describe techniques to allow the subcellular localization of pathogen effectors inside infected plant cells or tissues, based largely on infiltration of plant tissue by Agrobacterium tumefaciens and its delivery of DNA encoding fluorescent protein-tagged effectors, and subsequent confocal microscopy.

UR - http://www.scopus.com/inward/record.url?scp=79958253439&partnerID=8YFLogxK

U2 - 10.1007/978-1-61737-998-7_16

DO - 10.1007/978-1-61737-998-7_16

M3 - Chapter (peer-reviewed)

C2 - 21359810

AN - SCOPUS:79958253439

SN - 9781617379970

T3 - Methods in Molecular Biology

SP - 195

EP - 209

BT - Plant immunity

A2 - McDowell, John D.

PB - Humana Press

CY - New York

ER -

Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue

Abstract

Publication series

Access to Document

Other files and links

Fingerprint

Aref#d: 20586. What are the Roles of Oomycete RXLR Effectors in the Establishment of Plant Disease? (joint with Warwick University). Was CO0221

Cite this

Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue

Abstract

Publication series

Access to Document

Other files and links

Fingerprint

Projects

Aref#d: 20586. What are the Roles of Oomycete RXLR Effectors in the Establishment of Plant Disease? (joint with Warwick University). Was CO0221

Cite this